Pifu-xingbing zhenliaoxue zazhi (Apr 2019)

Effects of all-trans retinoic acid on the function of Cx43 and GJIC in HaCaT cells induced by IL-22

  • Xiao-dong LIANG,
  • Jie DENG,
  • Rui-xian YE,
  • Huan-yan LIU,
  • Qi-ying XIONG,
  • Ru-jun XUE,
  • Hui-heng CHEN,
  • Yu-mei LIU,
  • Jing-yao LIANG,
  • Xi-bao ZHANG

DOI
https://doi.org/10.3969/j.issn.1674-8468.2019.02.002
Journal volume & issue
Vol. 26, no. 2
pp. 67 – 72

Abstract

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Objective: To investigate the effect of all-trans retinoic acid (ATRA) on Cx43 protein expression and GJIC function in IL-22-treated HaCaT cells, and whether it is related to the JNK pathway to further elaborate molecular mechanism of ATRA on GJIC function. Methods: The optimal pretreated time affected by ATRA on HaCaT cells was screened in IL-22 treatment group.Scrape-loading dye-transfer assay was applied to observe the variation of GJIC function.The expression value of Cx43, p-JNK, JNK in ATRA and/or IL-22 treatment group were detected by immunofluorescence. Results: ATRA caused time-dependent increasing of GJIC function in HaCaT cells. After treatment of ATRA on IL-22 induced HaCaT cells, Cx43 protein expression level increased, GJIC function up-regulated, and p-JNK expression had not significant change. Conclusions: ATRA inhibited the decrease of Cx43 expression by IL-22-mediated and down-regulation of GJIC, and this process was not related to the JNK pathway.

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