Frontiers in Immunology (Jan 2023)
Type 2 and type 17 effector cells are increased in the duodenal mucosa but not peripheral blood of patients with functional dyspepsia
- Grace L. Burns,
- Grace L. Burns,
- Grace L. Burns,
- Jessica K. Bruce,
- Jessica K. Bruce,
- Jessica K. Bruce,
- Kyra Minahan,
- Kyra Minahan,
- Kyra Minahan,
- Andrea Mathe,
- Andrea Mathe,
- Thomas Fairlie,
- Thomas Fairlie,
- Thomas Fairlie,
- Raquel Cameron,
- Raquel Cameron,
- Raquel Cameron,
- Crystal Naudin,
- Crystal Naudin,
- Prema M. Nair,
- Prema M. Nair,
- Prema M. Nair,
- Michael D. E. Potter,
- Michael D. E. Potter,
- Michael D. E. Potter,
- Mudar Zand Irani,
- Mudar Zand Irani,
- Mudar Zand Irani,
- Steven Bollipo,
- Steven Bollipo,
- Robert Foster,
- Lay T. Gan,
- Ayesha Shah,
- Ayesha Shah,
- Ayesha Shah,
- Natasha A. Koloski,
- Natasha A. Koloski,
- Natasha A. Koloski,
- Paul S. Foster,
- Paul S. Foster,
- Jay C. Horvat,
- Jay C. Horvat,
- Martin Veysey,
- Martin Veysey,
- Gerald Holtmann,
- Gerald Holtmann,
- Gerald Holtmann,
- Nick Powell,
- Marjorie M. Walker,
- Marjorie M. Walker,
- Marjorie M. Walker,
- Nicholas J. Talley,
- Nicholas J. Talley,
- Nicholas J. Talley,
- Simon Keely,
- Simon Keely,
- Simon Keely
Affiliations
- Grace L. Burns
- College of Health, Medicine and Wellbeing, The University of Newcastle, Callaghan, NSW, Australia
- Grace L. Burns
- Immune Health Research Program, Hunter Medical Research Institute, New Lambton Heights, NSW, Australia
- Grace L. Burns
- National Health & Medical Research Council (NHMRC) Centre of Research Excellence in Digestive Health, University of Newcastle, Newcastle, NSW, Australia
- Jessica K. Bruce
- College of Health, Medicine and Wellbeing, The University of Newcastle, Callaghan, NSW, Australia
- Jessica K. Bruce
- Immune Health Research Program, Hunter Medical Research Institute, New Lambton Heights, NSW, Australia
- Jessica K. Bruce
- National Health & Medical Research Council (NHMRC) Centre of Research Excellence in Digestive Health, University of Newcastle, Newcastle, NSW, Australia
- Kyra Minahan
- College of Health, Medicine and Wellbeing, The University of Newcastle, Callaghan, NSW, Australia
- Kyra Minahan
- Immune Health Research Program, Hunter Medical Research Institute, New Lambton Heights, NSW, Australia
- Kyra Minahan
- National Health & Medical Research Council (NHMRC) Centre of Research Excellence in Digestive Health, University of Newcastle, Newcastle, NSW, Australia
- Andrea Mathe
- College of Health, Medicine and Wellbeing, The University of Newcastle, Callaghan, NSW, Australia
- Andrea Mathe
- Immune Health Research Program, Hunter Medical Research Institute, New Lambton Heights, NSW, Australia
- Thomas Fairlie
- National Health & Medical Research Council (NHMRC) Centre of Research Excellence in Digestive Health, University of Newcastle, Newcastle, NSW, Australia
- Thomas Fairlie
- Translational Research Institute, Brisbane, QLD, Australia
- Thomas Fairlie
- Department of Gastroenterology and Hepatology, Princess Alexandra Hospital, Brisbane, QLD, Australia
- Raquel Cameron
- College of Health, Medicine and Wellbeing, The University of Newcastle, Callaghan, NSW, Australia
- Raquel Cameron
- Immune Health Research Program, Hunter Medical Research Institute, New Lambton Heights, NSW, Australia
- Raquel Cameron
- National Health & Medical Research Council (NHMRC) Centre of Research Excellence in Digestive Health, University of Newcastle, Newcastle, NSW, Australia
- Crystal Naudin
- College of Health, Medicine and Wellbeing, The University of Newcastle, Callaghan, NSW, Australia
- Crystal Naudin
- Immune Health Research Program, Hunter Medical Research Institute, New Lambton Heights, NSW, Australia
- Prema M. Nair
- College of Health, Medicine and Wellbeing, The University of Newcastle, Callaghan, NSW, Australia
- Prema M. Nair
- Immune Health Research Program, Hunter Medical Research Institute, New Lambton Heights, NSW, Australia
- Prema M. Nair
- National Health & Medical Research Council (NHMRC) Centre of Research Excellence in Digestive Health, University of Newcastle, Newcastle, NSW, Australia
- Michael D. E. Potter
- College of Health, Medicine and Wellbeing, The University of Newcastle, Callaghan, NSW, Australia
- Michael D. E. Potter
- Immune Health Research Program, Hunter Medical Research Institute, New Lambton Heights, NSW, Australia
- Michael D. E. Potter
- National Health & Medical Research Council (NHMRC) Centre of Research Excellence in Digestive Health, University of Newcastle, Newcastle, NSW, Australia
- Mudar Zand Irani
- College of Health, Medicine and Wellbeing, The University of Newcastle, Callaghan, NSW, Australia
- Mudar Zand Irani
- Immune Health Research Program, Hunter Medical Research Institute, New Lambton Heights, NSW, Australia
- Mudar Zand Irani
- National Health & Medical Research Council (NHMRC) Centre of Research Excellence in Digestive Health, University of Newcastle, Newcastle, NSW, Australia
- Steven Bollipo
- College of Health, Medicine and Wellbeing, The University of Newcastle, Callaghan, NSW, Australia
- Steven Bollipo
- Department of Gastroenterology, John Hunter Hospital, Newcastle, NSW, Australia
- Robert Foster
- Department of Gastroenterology, John Hunter Hospital, Newcastle, NSW, Australia
- Lay T. Gan
- Department of Gastroenterology, John Hunter Hospital, Newcastle, NSW, Australia
- Ayesha Shah
- National Health & Medical Research Council (NHMRC) Centre of Research Excellence in Digestive Health, University of Newcastle, Newcastle, NSW, Australia
- Ayesha Shah
- Translational Research Institute, Brisbane, QLD, Australia
- Ayesha Shah
- Department of Gastroenterology and Hepatology, Princess Alexandra Hospital, Brisbane, QLD, Australia
- Natasha A. Koloski
- National Health & Medical Research Council (NHMRC) Centre of Research Excellence in Digestive Health, University of Newcastle, Newcastle, NSW, Australia
- Natasha A. Koloski
- Translational Research Institute, Brisbane, QLD, Australia
- Natasha A. Koloski
- Department of Gastroenterology and Hepatology, Princess Alexandra Hospital, Brisbane, QLD, Australia
- Paul S. Foster
- College of Health, Medicine and Wellbeing, The University of Newcastle, Callaghan, NSW, Australia
- Paul S. Foster
- Immune Health Research Program, Hunter Medical Research Institute, New Lambton Heights, NSW, Australia
- Jay C. Horvat
- College of Health, Medicine and Wellbeing, The University of Newcastle, Callaghan, NSW, Australia
- Jay C. Horvat
- Immune Health Research Program, Hunter Medical Research Institute, New Lambton Heights, NSW, Australia
- Martin Veysey
- College of Health, Medicine and Wellbeing, The University of Newcastle, Callaghan, NSW, Australia
- Martin Veysey
- Hull-York Medical School, University of Hull, Hull, United Kingdom
- Gerald Holtmann
- National Health & Medical Research Council (NHMRC) Centre of Research Excellence in Digestive Health, University of Newcastle, Newcastle, NSW, Australia
- Gerald Holtmann
- Translational Research Institute, Brisbane, QLD, Australia
- Gerald Holtmann
- Department of Gastroenterology and Hepatology, Princess Alexandra Hospital, Brisbane, QLD, Australia
- Nick Powell
- Faculty of Medicine, Imperial College London, London, United Kingdom
- Marjorie M. Walker
- College of Health, Medicine and Wellbeing, The University of Newcastle, Callaghan, NSW, Australia
- Marjorie M. Walker
- Immune Health Research Program, Hunter Medical Research Institute, New Lambton Heights, NSW, Australia
- Marjorie M. Walker
- National Health & Medical Research Council (NHMRC) Centre of Research Excellence in Digestive Health, University of Newcastle, Newcastle, NSW, Australia
- Nicholas J. Talley
- College of Health, Medicine and Wellbeing, The University of Newcastle, Callaghan, NSW, Australia
- Nicholas J. Talley
- Immune Health Research Program, Hunter Medical Research Institute, New Lambton Heights, NSW, Australia
- Nicholas J. Talley
- National Health & Medical Research Council (NHMRC) Centre of Research Excellence in Digestive Health, University of Newcastle, Newcastle, NSW, Australia
- Simon Keely
- College of Health, Medicine and Wellbeing, The University of Newcastle, Callaghan, NSW, Australia
- Simon Keely
- Immune Health Research Program, Hunter Medical Research Institute, New Lambton Heights, NSW, Australia
- Simon Keely
- National Health & Medical Research Council (NHMRC) Centre of Research Excellence in Digestive Health, University of Newcastle, Newcastle, NSW, Australia
- DOI
- https://doi.org/10.3389/fimmu.2022.1051632
- Journal volume & issue
-
Vol. 13
Abstract
BackgroundFunctional dyspepsia is characterised by chronic symptoms of post-prandial distress or epigastric pain not associated with defined structural pathology. Increased peripheral gut-homing T cells have been previously identified in patients. To date, it is unknown if these T cells were antigen-experienced, or if a specific phenotype was associated with FD.ObjectiveThis study aimed to characterise T cell populations in the blood and duodenal mucosa of FD patients that may be implicated in disease pathophysiology.MethodsWe identified duodenal T cell populations from 23 controls and 49 Rome III FD patients by flow cytometry using a surface marker antibody panel. We also analysed T cell populations in peripheral blood from 37 controls and 61 patients. Where available, we examined the number of duodenal eosinophils in patients and controls.ResultsThere was a shift in the duodenal T helper cell balance in FD patients compared to controls. For example, patients had increased duodenal mucosal Th2 populations in the effector (13.03 ± 16.11, 19.84 ± 15.51, p=0.038), central memory (23.75 ± 18.97, 37.52 ± 17.51, p=0.007) and effector memory (9.80±10.50 vs 20.53±14.15, p=0.001) populations. Th17 populations were also increased in the effector (31.74±24.73 vs 45.57±23.75, p=0.03) and effector memory (11.95±8.42 vs 18.44±15.63, p=0.027) subsets. Peripheral T cell populations were unchanged between FD and control.ConclusionOur findings identify an association between lymphocyte populations and FD, specifically a Th2 and Th17 signature in the duodenal mucosa. The presence of effector and memory cells suggest that the microinflammation in FD is antigen driven.
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