Photo-crosslinked bioactive BG/BMSCs@GelMA hydrogels for bone-defect repairs

Yufeng Ai; Fang Dai; Wenfeng Li; Fancheng Xu; Hanwen Yang; Jianxin Wu; Kaiqiang Yang; Li Li; Fanrong Ai; Li Song

Materials Today Bio (Dec 2023)

Photo-crosslinked bioactive BG/BMSCs@GelMA hydrogels for bone-defect repairs

Yufeng Ai,
Fang Dai,
Wenfeng Li,
Fancheng Xu,
Hanwen Yang,
Jianxin Wu,
Kaiqiang Yang,
Li Li,
Fanrong Ai,
Li Song

Affiliations

Yufeng Ai: Center of Stomatology, The Second Affiliated Hospital of Nanchang University, Nanchang, Jiangxi, 33006, China; The Institute of Periodontal Disease, Nanchang University, Nanchang, Jiangxi, 33006, China; JXHC Key Laboratory of Periodontology (The Second Affiliated Hospital of Nanchang University), Nanchang, Jiangxi, 33006, China; The Second Clinical Medical School, Nanchang University, Nanchang, Jiangxi, 33006, China
Fang Dai: Center of Stomatology, The Second Affiliated Hospital of Nanchang University, Nanchang, Jiangxi, 33006, China; The Institute of Periodontal Disease, Nanchang University, Nanchang, Jiangxi, 33006, China; JXHC Key Laboratory of Periodontology (The Second Affiliated Hospital of Nanchang University), Nanchang, Jiangxi, 33006, China
Wenfeng Li: Center of Stomatology, The Second Affiliated Hospital of Nanchang University, Nanchang, Jiangxi, 33006, China; The Institute of Periodontal Disease, Nanchang University, Nanchang, Jiangxi, 33006, China; JXHC Key Laboratory of Periodontology (The Second Affiliated Hospital of Nanchang University), Nanchang, Jiangxi, 33006, China
Fancheng Xu: Center of Stomatology, The Second Affiliated Hospital of Nanchang University, Nanchang, Jiangxi, 33006, China; The Institute of Periodontal Disease, Nanchang University, Nanchang, Jiangxi, 33006, China; JXHC Key Laboratory of Periodontology (The Second Affiliated Hospital of Nanchang University), Nanchang, Jiangxi, 33006, China; The Second Clinical Medical School, Nanchang University, Nanchang, Jiangxi, 33006, China
Hanwen Yang: The Second Clinical Medical School, Nanchang University, Nanchang, Jiangxi, 33006, China
Jianxin Wu: Center of Stomatology, The Second Affiliated Hospital of Nanchang University, Nanchang, Jiangxi, 33006, China; The Institute of Periodontal Disease, Nanchang University, Nanchang, Jiangxi, 33006, China; JXHC Key Laboratory of Periodontology (The Second Affiliated Hospital of Nanchang University), Nanchang, Jiangxi, 33006, China; The Second Clinical Medical School, Nanchang University, Nanchang, Jiangxi, 33006, China
Kaiqiang Yang: Center of Stomatology, The Second Affiliated Hospital of Nanchang University, Nanchang, Jiangxi, 33006, China; The Institute of Periodontal Disease, Nanchang University, Nanchang, Jiangxi, 33006, China; JXHC Key Laboratory of Periodontology (The Second Affiliated Hospital of Nanchang University), Nanchang, Jiangxi, 33006, China; The Second Clinical Medical School, Nanchang University, Nanchang, Jiangxi, 33006, China
Li Li: Center of Stomatology, The Second Affiliated Hospital of Nanchang University, Nanchang, Jiangxi, 33006, China; The Institute of Periodontal Disease, Nanchang University, Nanchang, Jiangxi, 33006, China; JXHC Key Laboratory of Periodontology (The Second Affiliated Hospital of Nanchang University), Nanchang, Jiangxi, 33006, China; The Second Clinical Medical School, Nanchang University, Nanchang, Jiangxi, 33006, China
Fanrong Ai: School of Advanced Manufacturing, Nanchang University, Nanchang, Jiangxi, 33006, China; Corresponding author.
Li Song: Center of Stomatology, The Second Affiliated Hospital of Nanchang University, Nanchang, Jiangxi, 33006, China; The Institute of Periodontal Disease, Nanchang University, Nanchang, Jiangxi, 33006, China; JXHC Key Laboratory of Periodontology (The Second Affiliated Hospital of Nanchang University), Nanchang, Jiangxi, 33006, China; Corresponding author. Center of Stomatology, The Second Affiliated Hospital of Nanchang University, Nanchang, Jiangxi, 33006, China.

Journal volume & issue: Vol. 23
p. 100882

Abstract

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The clinical treatments of bone defects remain a challenge. Hydrogels containing bone marrow mesenchymal stem cells (BMSCs) are extensively used to bone regeneration because of excellent biocompatibility and hydrophilicity. However, the insufficient osteo-induction capacity of the BMSC-loaded hydrogels limits their clinical applications. In this study, bio-active glass (BG) and BMSCs were combined with gelatin methacryloyl (GelMA) to fabricate composite hydrogels via photo-crosslinking, and the regulation of bone regeneration was investigated. In vitro experiments showed that the BG/BMSCs@GelMA hydrogel had excellent cytocompatibility and promoted osteogenic differentiation in BMSCs. Furthermore, the BG/BMSCs@GelMA hydrogel was injected into critical-sized calvarial defects, and the results further confirmed its excellent angiogenetic and bone regeneration capacity. In addition, BG/BMSCs@GelMA promoted the polarization of macrophages towards the M2 phenotype. In summary, this novel composite hydrogel demonstrated remarkable potential for application in bone regeneration due to its immunomodulatory, excellent angiogenetic as well as osteo-induction capacity.

Published in Materials Today Bio

ISSN: 2590-0064 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Medicine: Medicine (General); Science: Biology (General)
Website: https://www.journals.elsevier.com/materials-today-bio

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