Determination of clofibrate in biological fluids by thin-layer and gas—liquid chromatography

Abstract

A specific and sensitive method is described for the detection of clofibrate in biological fluids. The drug is separated from associated fatty acids by thin-layer chromatography and the methyl ester is quantified by gas—liquid chromatography. Recovery is excellent, and any small losses are corrected with an internal recovery standard. Although more time-consuming than other available techniques, the method offers advantages for accurate studies of clofibrate metabolism.

Keywords

• p-chlorophenoxyisobutyric acid
• internal standards
• tritiated clofibrate