Nature Communications (Mar 2018)

uvCLAP is a fast and non-radioactive method to identify in vivo targets of RNA-binding proteins

  • Daniel Maticzka,
  • Ibrahim Avsar Ilik,
  • Tugce Aktas,
  • Rolf Backofen,
  • Asifa Akhtar

DOI
https://doi.org/10.1038/s41467-018-03575-4
Journal volume & issue
Vol. 9, no. 1
pp. 1 – 13

Abstract

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RNA-binding proteins have important roles in gene expression and regulation. Here the authors develop uvCLAP to purify proteins and determine their binding sites without the use of radioactivity.