Detection of spacer precursors formed in vivo during primed CRISPR adaptation

Anna A. Shiriaeva; Ekaterina Savitskaya; Kirill A. Datsenko; Irina O. Vvedenskaya; Iana Fedorova; Natalia Morozova; Anastasia Metlitskaya; Anton Sabantsev; Bryce E. Nickels; Konstantin Severinov; Ekaterina Semenova

doi:10.1038/s41467-019-12417-w

Nature Communications (Oct 2019)

Detection of spacer precursors formed in vivo during primed CRISPR adaptation

Anna A. Shiriaeva,
Ekaterina Savitskaya,
Kirill A. Datsenko,
Irina O. Vvedenskaya,
Iana Fedorova,
Natalia Morozova,
Anastasia Metlitskaya,
Anton Sabantsev,
Bryce E. Nickels,
Konstantin Severinov,
Ekaterina Semenova

Affiliations

Anna A. Shiriaeva: Center of Life Sciences, Skolkovo Institute of Science and Technology
Ekaterina Savitskaya: Center of Life Sciences, Skolkovo Institute of Science and Technology
Kirill A. Datsenko: Department of Molecular Biology and Biochemistry, Waksman Institute, Rutgers University
Irina O. Vvedenskaya: Department of Genetics, Waksman Institute, Rutgers University
Iana Fedorova: Center of Life Sciences, Skolkovo Institute of Science and Technology
Natalia Morozova: Center of Life Sciences, Skolkovo Institute of Science and Technology
Anastasia Metlitskaya: Institute of Molecular Genetics, Russian Academy of Sciences
Anton Sabantsev: Peter the Great St. Petersburg Polytechnic University
Bryce E. Nickels: Department of Genetics, Waksman Institute, Rutgers University
Konstantin Severinov: Center of Life Sciences, Skolkovo Institute of Science and Technology
Ekaterina Semenova: Department of Molecular Biology and Biochemistry, Waksman Institute, Rutgers University

DOI: https://doi.org/10.1038/s41467-019-12417-w
Journal volume & issue: Vol. 10, no. 1
pp. 1 – 9

Abstract

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Primed adaptation in the CRISPR-Cas system helps recognition of previously encountered sequence elements and promotes the formation of new memories. Here the authors characterized spacer precursors of type I-E and type I-F CRISPR-Cas system using in vivo models.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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