Kasmera (Jun 2006)
Comparison Between Inmunochromatographic and Enzyme Linked Immunosorbent Assay (ELISA) in the Dengue Diagnosis
Abstract
The search for techniques for the diagnostic confirmation of Dengue (IgM and IgG) and recent commercialization of such serums requires of the study of their sensibility, specificity, reproductibility, speed and costs. For the purpose of comparing two serological techniques for the diagnosis of this infection, 184 serum samples of patients with confirmed diagnosis of Dengue and 60 with Rubella, were analyzed by immune-chromatographic (IC) and enzyme linked immune-absorbent tests (ELISA) by determination of anti-dengue antibody IgM and IgG. In the IC and ELISA techniques levels of 0,70 and 0,79 in nosologic sensibility were obtained, respectively in the determination of antibody IgM; while the sensibility of ELISA IgG resulted significantly elevated (p<0,0001) with respect to IC (0,92 vs 0,65). Both techniques reached reproduction levels of 98% and a specificity of 100%. These results suggest that ELISA is best for the detection of primary and secondary infections, while IC was the fastest assay and applicable in outbreaks and epidemics; however, it was less sensitive for the diagnosis of secondary infections for dengue virus and consequently less adequate for the screening of dengue hemorrhagic fever and dengue shock syndrome (DHF/DSS) in endemic areas.
