In situ structure and assembly of the multidrug efflux pump AcrAB-TolC

Xiaodong Shi; Muyuan Chen; Zhili Yu; James M. Bell; Hans Wang; Isaac Forrester; Heather Villarreal; Joanita Jakana; Dijun Du; Ben F. Luisi; Steven J. Ludtke; Zhao Wang

doi:10.1038/s41467-019-10512-6

Nature Communications (Jun 2019)

In situ structure and assembly of the multidrug efflux pump AcrAB-TolC

Xiaodong Shi,
Muyuan Chen,
Zhili Yu,
James M. Bell,
Hans Wang,
Isaac Forrester,
Heather Villarreal,
Joanita Jakana,
Dijun Du,
Ben F. Luisi,
Steven J. Ludtke,
Zhao Wang

Affiliations

Xiaodong Shi: Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine
Muyuan Chen: Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine
Zhili Yu: Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine
James M. Bell: Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine
Hans Wang: Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine
Isaac Forrester: CryoEM Core at Baylor College of Medicine
Heather Villarreal: CryoEM Core at Baylor College of Medicine
Joanita Jakana: CryoEM Core at Baylor College of Medicine
Dijun Du: Department of Biochemistry, University of Cambridge
Ben F. Luisi: Department of Biochemistry, University of Cambridge
Steven J. Ludtke: Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine
Zhao Wang: Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine

DOI: https://doi.org/10.1038/s41467-019-10512-6
Journal volume & issue: Vol. 10, no. 1
pp. 1 – 6

Abstract

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Multidrug efflux pumps actively expel a wide range of toxic substrates from bacteria and play a major role in drug resistance. Here authors show the in situ structure of the efflux pump AcrAB-TolC obtained by electron cryo-tomography and subtomogram averaging.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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