Protocol to establish a genetically engineered mouse model of IDH1-mutant astrocytoma

Diana D. Shi; Joyce H. Lee; Adam C. Wang; Januka Khanal; Wenhua Gao; William G. Kaelin, Jr.; Samuel K. McBrayer

STAR Protocols (Jun 2023)

Protocol to establish a genetically engineered mouse model of IDH1-mutant astrocytoma

Diana D. Shi,
Joyce H. Lee,
Adam C. Wang,
Januka Khanal,
Wenhua Gao,
William G. Kaelin, Jr.,
Samuel K. McBrayer

Affiliations

Diana D. Shi: Department of Radiation Oncology, Dana-Farber/Brigham and Women’s Cancer Center, Harvard Medical School, Boston, MA 02215, USA; Children’s Medical Center Research Institute, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA; Corresponding author
Joyce H. Lee: Department of Medical Oncology, Dana-Farber Cancer Institute and Brigham and Women’s Hospital, Harvard Medical School, Boston, MA 02215, USA
Adam C. Wang: Department of Medical Oncology, Dana-Farber Cancer Institute and Brigham and Women’s Hospital, Harvard Medical School, Boston, MA 02215, USA
Januka Khanal: Department of Medical Oncology, Dana-Farber Cancer Institute and Brigham and Women’s Hospital, Harvard Medical School, Boston, MA 02215, USA
Wenhua Gao: Department of Medical Oncology, Dana-Farber Cancer Institute and Brigham and Women’s Hospital, Harvard Medical School, Boston, MA 02215, USA
William G. Kaelin, Jr.: Department of Medical Oncology, Dana-Farber Cancer Institute and Brigham and Women’s Hospital, Harvard Medical School, Boston, MA 02215, USA; Howard Hughes Medical Institute, Chevy Chase, MD 20815, USA
Samuel K. McBrayer: Children’s Medical Center Research Institute, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA; Department of Pediatrics, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA; Harold C. Simmons Comprehensive Cancer Center, University of Texas Southwestern Medical Center, Dallas, TX 75235, USA; Corresponding author

Journal volume & issue: Vol. 4, no. 2
p. 102281

Abstract

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Summary: Lower-grade gliomas exhibit a high prevalence of isocitrate dehydrogenase 1 (IDH1) mutations, but faithful models for studying these tumors are lacking. Here, we present a protocol to establish a genetically engineered mouse (GEM) model of grade 3 astrocytoma driven by the Idh1R132H oncogene. We describe steps for breeding compound transgenic mice and intracranially delivering adeno-associated virus particles, followed by post-surgical surveillance via magnetic resonance imaging. This protocol enables the generation and use of a GEM to study lower-grade IDH-mutant gliomas.For complete details on the use and execution of this protocol, please refer to Shi et al. (2022).1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.

Published in STAR Protocols

ISSN: 2666-1667 (Online)
Publisher: Elsevier
Country of publisher: United States
LCC subjects: Science: Science (General)
Website: https://star-protocols.cell.com/

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Abstract

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