Apoptotic Genes’ Expression in an Ovarian Cancer Cell Line (A2780) Exposed to Green Synthesized Cerium Oxide Nanoemulsion

Abstract

Introduction: Cancer can be defined as an illness of reformed gene expression. There are numerous agents affecting gene expression and altering cellular activities. Nanotechnology has offered the possibility of modulating tumor suppressor genes’ expression, improving kinetics of gene-targeted therapeutics, and simplifying drug delivery to tumors and across bio-complexes.Methods: Gene expressions of caspase3, 8 and 9, Bax and Bcl2 were assessed after RNA extraction and cDNA synthesis by real-time polymerase chain reaction (PCR) in an ovarian cancerous cell line (A2780). Real-time PCR was employed to determine the change fold of apoptotic genes in the cells exposed to the biosynthesized CeO2-NPs (cerium oxide nanoparticles) at the dose of 0, 7, 9, and 11 μg/mL after 24 hours of incubation. Results: Our findings displayed a significant increase in the antioxidant genes’ expression in the A2870 cells. The results exhibited that the biosynthesized CeO2-NPs could enhance the Bax/Bcl2 ratio in a dose-dependent way. Also, the expression of caspase3, 8, and 9 up-regulated significantly under the dose of 11 μg/mL.Conclusion: Considering the effects of the bio-fabricated CeO2-NPs on the expression of apoptotic genes in ovarian cancerous cell lines, these nanoparticles (NPs) may be employed in pharmacology to develop new anti-cancer medications.

Keywords

• cerium oxide nanoemulsion
• apoptotic gene
• ovarian cancer cell line a2780