Endoflas FS Decreases the Viability and Mineralisation Process in Human Alveolar Osteoblastic Cells

Abstract

Introduction: Endoflas FS, a root canal filling material, is indicated for the endodontic management of deciduous teeth. Several studies have shown undesirable effects of Endoflas FS on periodontal tissues. It is important to determine the possible toxic effects of Endoflas FS on osteoblast cells, as a small amount of microfiltration may exist following obturation of the root canal resulting in a direct contact with bone cells. Aim: To evaluate the effect of different amounts of Endoflas FS on viability, mineralisation and production of TNF-α and IL-6 in a human alveolar osteoblastic cell line. Materials and Methods: The present in vitro study was done using a human alveolar osteoblastic cell line which was exposed to different amounts of Endoflas FS. The MTT assay was performed to determine the cell viability (2-24 hours). Mineralisation was evaluated by red alizarin at 7-14 days. The production of TNF-α and IL-6 was determined by EnzymeLinked Immunosorbent Assay (ELISA) at 12 and 24 hours. Results: At high amounts Endoflas FS decreased the viability of osteoblasts and reduced the formation of mineralisation nodules and the production of IL-6 and TNF-α. Conclusion: At low amounts Endoflas FS may not generate changes in osteoblastic cells; however, at high amounts Endolfas FS may be toxic, decreasing cell viability and mineralisation.

Keywords

• bone cells
• cytotoxicity
• mineral matrix
• root canal sealer