Changes in cytokines in platelet-rich plasma after short-term cryopreservation at -80 ℃

Abstract

Objective To analyze the changes in important indicators such as P-selectin, vascular endothelial growth factor (VEGF), platelet-derived growth factor (PDGF), and pH value in platelet-rich plasma (PRP) after short-term cryopreservation at -80 ℃, and to provide a theoretical basis for the low-temperature storage of PRP. Methods Each of PRP samples prepared from the blood from 30 healthy donors was divided into 5 aliquots, with 0.5 mL retained per aliquot. These samples were de-signated as group A (fresh sample) and groups B to E (cryopreservation for 5, 10, 15, and 20 d, respectively). The pH values of the five groups were measured. The concentrations of P-selectin, PDGF, and VEGF in each group were determined by the ELISA method. The effect of groups A and E on the vascular formation capacity of human umbilical vein endothelial cells (HUVEC) was determined by the angiogenesis assay. Results No significant differences were observed in the concentrations of P-selectin, PDGF, and VEGF as well as the pH values between group A and groups B to E (P >0.05). Compared with the fresh sample (group A), the number of vessels formed from HUVECs in the sample frozen for 20 d (group E) was basically the same, with no significant differences (P>0.05). Conclusion The cryopreservation of PRP at -80 ℃ has no significant effects on its pH value and concentrations of P-selectin, VEGF, and PDGF, thus extending the clinical application time of PRP.

Keywords

• platelet-rich plasma|cryopreservation|p-selectin|vascular endothelial growth factors|platelet-derived growth factor|hydrogen-ion concentration