Pleurocidin Peptide Enhances Grouper Anti-Vibrio harveyi Immunity Elicited by Poly(lactide-co-glycolide)-Encapsulated Recombinant Glyceraldehyde-3-phosphate Dehydrogenase

Shu-Chun Chuang; Wan-Ling Huang; Sau-Wei Kau; Yun-Pei Yang; Chung-Da Yang

doi:10.3390/vaccines2020380

Vaccines (May 2014)

Pleurocidin Peptide Enhances Grouper Anti-Vibrio harveyi Immunity Elicited by Poly(lactide-co-glycolide)-Encapsulated Recombinant Glyceraldehyde-3-phosphate Dehydrogenase

Shu-Chun Chuang,
Wan-Ling Huang,
Sau-Wei Kau,
Yun-Pei Yang,
Chung-Da Yang

Affiliations

Shu-Chun Chuang: Department of Physiology, College of Medicine, Kaohsiung Medical University, No. 100, Shih-Chuan 1st Road, Kaohsiung 807, Taiwan
Wan-Ling Huang: Graduate Institute of Animal Vaccine Technology, National Pingtung University of Science and Technology, No. 1, Shuefu Road, Neipu, Pingtung 912, Taiwan
Sau-Wei Kau: Graduate Institute of Animal Vaccine Technology, National Pingtung University of Science and Technology, No. 1, Shuefu Road, Neipu, Pingtung 912, Taiwan
Yun-Pei Yang: Graduate Institute of Animal Vaccine Technology, National Pingtung University of Science and Technology, No. 1, Shuefu Road, Neipu, Pingtung 912, Taiwan
Chung-Da Yang: Graduate Institute of Animal Vaccine Technology, National Pingtung University of Science and Technology, No. 1, Shuefu Road, Neipu, Pingtung 912, Taiwan

DOI: https://doi.org/10.3390/vaccines2020380
Journal volume & issue: Vol. 2, no. 2
pp. 380 – 396

Abstract

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Outer membrane proteins, such as glyceraldehyde-3-phosphate dehydrogenase (GAPDH), are considered immunodominant antigens for eliciting protective immunity against Vibrio harveyi, the main etiological agent of vibriosis in fish. Cationic antimicrobial peptides (AMPs), such as pleurocidin (PLE), play important roles in activating and recruiting immune cells, thereby contributing to subsequent innate and adaptive immune responses. In the present study, we aimed to use PLE peptide as a potent adjuvant to improve the immunogenicity of V. harveyi recombinant GAPDH (rGAPDH). In order to prepare a controlled-release vaccine, PLE peptide and rGAPDH protein were simultaneously encapsulated into polymeric microparticles made from the biodegradable poly(lactide-co-glycolide) (PLG) polymer. The resulting PLG-encapsulated PLE plus rGAPDH (PLG-PLE/rGAPDH) microparticles, 3.21–6.27 μm in diameter, showed 72%–83% entrapment efficiency and durably released both PLE and rGAPDH for a long 30-day period. Following peritoneal immunization in grouper (Epinephelus coioides), PLG-PLE/rGAPDH microparticles resulted in significantly higher (p < 0.05, nested design) long-lasting GAPDH-specific immunity (serum titers and lymphocyte proliferation) than PLG-encapsulated rGAPDH (PLG-rGAPDH) microparticles. After an experimental challenge of V. harveyi, PLG-PLE/rGAPDH microparticles conferred a high survival rate (85%), which was significantly higher (p < 0.05, chi-square test) than that induced by PLG-rGAPDH microparticles (67%). In conclusion, PLE peptide exhibits an efficacious adjuvant effect to elicit not only improved immunity, but also enhanced protection against V. harveyi in grouper induced by rGAPDH protein encapsulated in PLG microparticles.

Published in Vaccines

ISSN: 2076-393X (Online)
Publisher: MDPI AG
Country of publisher: Switzerland
LCC subjects: Medicine
Website: http://www.mdpi.com/journal/vaccines

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