Effects of Combination of Water-Retaining Agent and Nitrogen Fertilizer on Soil Characteristics and Growth of Winter Wheat under Subsoiling Tillage in South Loess Plateau of China

Abstract

This study was carried out to evaluate the effects of the combined application of water-retaining agents and nitrogen fertilizers on soil physicochemical properties, bacterial communities, and root growth under winter wheat planting mode in the Guanzhong area of Shaanxi Province. Based on the positioning experiment of dry farming in the loessal soil area of Shaanxi Province, four treatments were set up by using the tillage method of subsoiling + rotary tillage and straw returning: only fertilization (U), only water retention agent sodium polyacrylate (C3H3NaO2)n (S), combined use of water-retaining agent sodium polyacrylate (C3H3NaO2)n and fertilizer (US), and control group CK (no treatment). The ultra-high-throughput sequencing of 16S rRNA genes of soil bacteria was performed by the Illumina Hiseq platform, and the effects of different tillage measures on soil bacterial diversity and community structure were analyzed. In addition, the effects of these tillage measures on soil physicochemical properties and winter wheat root length density at booting and flowering stages were evaluated. The results indicated that the combination of the water-retaining agent and fertilizer markedly enhanced the contents of ammonium nitrogen, available phosphorus, and available potassium in the 0~20 cm soil layer, significantly increased the soil moisture content, and promoted the deep growth of roots. The root length density was 4.70 times higher than that of the control group at the booting stage. In addition, the combined application alleviated the decrease in soil microbial diversity caused by individual fertilization, especially significantly increasing the abundance of Gemmatimonadetes, Acidobacteria, and Planctomycetes in the 0~10 cm soil layer. This study reveals the potential of the combined use of water retention agents and fertilizers to optimize the soil environment and enhance winter wheat yield, which provides a scientific basis for improving local agricultural practices.

Keywords

• subsoiling
• water-retaining agent
• nitrogen fertilizer
• soil physicochemical properties
• bacterial community structure