Determination of the Relationship of Serum Amino Acid Profile with Sex and Body Weight in Healthy Geese by Liquid Chromatography-Tandem Mass Spectrometry

Abstract

ABSTRACT The aim of this study is to determine the serum amino acid profile using Liquid Chromatography-Tandem Mass Spectrometry (LC-MS / MS) in healthy male and female geese of the same age that were raised in similar care and feeding conditions. The animal material of the study consisted of a total of 41 geese, 18 male, and 23 female of the same age (9 months). After a 12h fasting period of the geese, blood samples were taken from V. subcutanaeulnaris into tubes without anticoagulant. After separating the serums, the samples were preserved at -20 O C degrees until Methylglutaryl (Met-Glu), Valine (Val), Leucyl-Isoleucine (Leu-Ile), Methionine (Met), Phenylalanine (Phe), Argininosuccinate (ASA), Tyrosine (Tyr), Aspartic acid (Asp), Alanine (Ala), Arginine (Arg), Citrulline (Cit), Glycine (Gly), Ornithine (Orn), Glutamic acid (Glu) were analyzed. The Val, Asp, Arg, Cit, Gly, and Orn levels of male geese were higher compared to female geese in the research (p<0.05). It was determined that Asp, Arg, Cit, and Gly levels increased as the body weights of the geese increased (p<0.05). It was also determined that the effect of Gender x Body Weight interaction on Val, Cit, and Orn levels was significant (p<0.05). As a result; it has been concluded that the serum amino acid profile of healthy geese can vary according to gender and live weight, and more studies are needed to elucidate the reasons for these changes.

Keywords

• Amino Acid Profile
• Goose
• LC-MS / MS