Single-cell transcriptome analysis of diffuse large B cells in cerebrospinal fluid of central nervous system lymphoma

Haoyu Ruan; Zhe Wang; Yue Zhai; Ying Xu; Linyu Pi; Jihong Zheng; Yihang Zhou; Cong Zhang; Ruofan Huang; Kun Chen; Xiangyu Li; Weizhe Ma; Zhiyuan Wu; Jie Shen; Xuan Deng; Chao Zhang; Ming Guan

iScience (Sep 2021)

Single-cell transcriptome analysis of diffuse large B cells in cerebrospinal fluid of central nervous system lymphoma

Haoyu Ruan,
Zhe Wang,
Yue Zhai,
Ying Xu,
Linyu Pi,
Jihong Zheng,
Yihang Zhou,
Cong Zhang,
Ruofan Huang,
Kun Chen,
Xiangyu Li,
Weizhe Ma,
Zhiyuan Wu,
Jie Shen,
Xuan Deng,
Chao Zhang,
Ming Guan

Affiliations

Haoyu Ruan: Department of Clinical Laboratory, Huashan Hospital, Fudan University, Shanghai, China; Department of Laboratory Medicine, the First Affiliated Hospital of Nanjing Medical University, Nanjing, China
Zhe Wang: Department of Plastic and Reconstructive Surgery, Shanghai Institute of Precision Medicine, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China
Yue Zhai: Translational Medical Center for Stem Cell Therapy and Institute for Regenerative Medicine, Shanghai East Hospital, Shanghai Key Laboratory of Signaling and Disease Research, Frontier Science Center for Stem Cell Research, School of Life Sciences and Technology, Tongji University, Shanghai, China
Ying Xu: Translational Medical Center for Stem Cell Therapy and Institute for Regenerative Medicine, Shanghai East Hospital, Shanghai Key Laboratory of Signaling and Disease Research, Frontier Science Center for Stem Cell Research, School of Life Sciences and Technology, Tongji University, Shanghai, China
Linyu Pi: Translational Medical Center for Stem Cell Therapy and Institute for Regenerative Medicine, Shanghai East Hospital, Shanghai Key Laboratory of Signaling and Disease Research, Frontier Science Center for Stem Cell Research, School of Life Sciences and Technology, Tongji University, Shanghai, China
Jihong Zheng: Translational Medical Center for Stem Cell Therapy and Institute for Regenerative Medicine, Shanghai East Hospital, Shanghai Key Laboratory of Signaling and Disease Research, Frontier Science Center for Stem Cell Research, School of Life Sciences and Technology, Tongji University, Shanghai, China
Yihang Zhou: Translational Medical Center for Stem Cell Therapy and Institute for Regenerative Medicine, Shanghai East Hospital, Shanghai Key Laboratory of Signaling and Disease Research, Frontier Science Center for Stem Cell Research, School of Life Sciences and Technology, Tongji University, Shanghai, China
Cong Zhang: Department of Plastic and Reconstructive Surgery, Shanghai Institute of Precision Medicine, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China
Ruofan Huang: Department of Oncology, Huashan Hospital, Fudan University, Shanghai, China
Kun Chen: Department of Clinical Laboratory, Huashan Hospital North, Fudan University, Shanghai, China
Xiangyu Li: Department of Clinical Laboratory, Huashan Hospital North, Fudan University, Shanghai, China
Weizhe Ma: Central Laboratory, Huashan Hospital, Fudan University, Shanghai, China
Zhiyuan Wu: Department of Clinical Laboratory, Huashan Hospital North, Fudan University, Shanghai, China
Jie Shen: 10K Genomics Technology Co.,Ltd., 333 Guiping Road, Shanghai 200233, China
Xuan Deng: Department of Clinical Laboratory, Huashan Hospital, Fudan University, Shanghai, China
Chao Zhang: Department of Plastic and Reconstructive Surgery, Shanghai Institute of Precision Medicine, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China; Corresponding author
Ming Guan: Department of Clinical Laboratory, Huashan Hospital, Fudan University, Shanghai, China; Corresponding author

Journal volume & issue: Vol. 24, no. 9
p. 102972

Abstract

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Summary: Diffuse large B cells in the cerebrospinal fluid (CSF-DLBCs) have offered great promise for the diagnostics and therapeutics of central nervous system lymphoma (CNSL) leptomeningeal involvement. To explore the phenotypic states of CSF-DLBCs, we analyzed the transcriptomes of more than one thousand CSF-DLBCs from six patients with CNSL diffuse large B-cell lymphoma (DLBCL) using Smart-seq2 single-cell RNA sequencing. CSF-DLBCs were defined based on abundant expression of B-cell markers, the active cell proliferation and energy metabolism properties, and immunoglobulin light chain restriction. We identified inherent heterogeneity of CSF-DLBCs, which were mainly manifested in cell cycle state, cancer-testis antigen expression, and classification based on single-cell germinal center B-cell signature. In addition, the 16 upregulated genes in CSF-DLBCs compared to various normal B cells showed great possibility in the homing effect of the CNS-DLBCL for the leptomeninges. Our results will provide insight into the mechanism research and diagnostic direction of CNSL-DLBCL leptomeningeal involvement.

Published in iScience

ISSN: 2589-0042 (Online)
Publisher: Elsevier
Country of publisher: United States
LCC subjects: Science
Website: http://www.cell.com/iscience/home

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