Italian Journal of Agronomy (Mar 2007)
Genetic Variations of Gliadin and HMW Glutenin Subunits in Spelt Wheat
Abstract
Gliadin and HMW glutenin subunit variations in 162 spelt wheat (Triticum spelta L.) accessions were detected by A-PAGE and SDS-PAGE. Higher gliadin variation was observed, and 121 gliadin patterns were detected. A total of 14 HMW-GS alleles were found. There were 2, 5 and 2 alleles at Glu-A1, Glu-B1 and Glu-D1 loci, respectively, resulting in 8 HMW-GS combinations. HMW-GS combination (1, 6+8, 2+12) was the dominant phenotype, which was found in 83.00% of the spelt wheat accessions. These results suggested that the polymorphism of spelt wheat on Gli-1 loci was higher than that on Glu-1 loci.
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