Efficient and Stable Magnetic Chitosan-Lipase B from <i>Candida Antarctica</i> Bioconjugates in the Enzymatic Kinetic Resolution of Racemic Heteroarylethanols

Abstract

Lipase B from Candida antarctica immobilized by covalent binding on sebacoyl-activated chitosan-coated magnetic nanoparticles proved to be an efficient biocatalyst (49.2−50% conversion in 3−16 h and >96% enantiomeric excess) for the enzymatic kinetic resolution of some racemic heteroarylethanols through transesterification with vinyl acetate. Under optimal conditions (vinyl acetate, n-hexane, 45 °C), the biocatalyst remains active after 10 cycles.

Keywords

• lipase b from <i>candida antarctica</i>
• magnetic nanoparticle
• chitosan
• covalent immobilization
• enzymatic kinetic resolution