Human breastmilk-derived Bifidobacterium longum subsp. infantis CCFM1269 regulates bone formation by the GH/IGF axis through PI3K/AKT pathway

Mengfan Ding; Bowen Li; Haiqin Chen; Dong Liang; R. Paul Ross; Catherine Stanton; Jianxin Zhao; Wei Chen; Bo Yang

doi:10.1080/19490976.2023.2290344

Gut Microbes (Dec 2024)

Human breastmilk-derived Bifidobacterium longum subsp. infantis CCFM1269 regulates bone formation by the GH/IGF axis through PI3K/AKT pathway

Mengfan Ding,
Bowen Li,
Haiqin Chen,
Dong Liang,
R. Paul Ross,
Catherine Stanton,
Jianxin Zhao,
Wei Chen,
Bo Yang

Affiliations

Mengfan Ding: State Key Laboratory of Food Science and Resources, Jiangnan University, Wuxi, Jiangsu, China
Bowen Li: State Key Laboratory of Food Science and Resources, Jiangnan University, Wuxi, Jiangsu, China
Haiqin Chen: State Key Laboratory of Food Science and Resources, Jiangnan University, Wuxi, Jiangsu, China
Dong Liang: Department of Applied Nutrition I, China National Center for Food Safety Risk Assessment, Beijing, China
R. Paul Ross: International Joint Research Center for Probiotics & Gut Health, Jiangnan University, Wuxi, Jiangsu, China
Catherine Stanton: International Joint Research Center for Probiotics & Gut Health, Jiangnan University, Wuxi, Jiangsu, China
Jianxin Zhao: State Key Laboratory of Food Science and Resources, Jiangnan University, Wuxi, Jiangsu, China
Wei Chen: State Key Laboratory of Food Science and Resources, Jiangnan University, Wuxi, Jiangsu, China
Bo Yang: State Key Laboratory of Food Science and Resources, Jiangnan University, Wuxi, Jiangsu, China

DOI: https://doi.org/10.1080/19490976.2023.2290344
Journal volume & issue: Vol. 16, no. 1

Abstract

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ABSTRACTBifidobacterium longum subsp. infantis is a prevalent member of the gut microbiota of breastfed infants. In this study, the effects of human breastmilk-derived B.longum subsp. infantis CCFM1269 on bone formation in developing BALB/c mice were investigated. Newborn female and male mice were assigned to control group (administered saline), CCFM1269 group (administered B. longum subsp. infantis CCFM1269, 1 × 109 CFU/mouse/day) and I5TI group (administered B. longum subsp. infantis I5TI, 1 × 109 CFU/mouse/day) from 1-week-old to 3-, 4- and 5-week old. B. longum subsp. infantis I5TI served as a negative control in this study. The results demonstrated that B. longum subsp. infantis CCFM1269 promoted bone formation in growing mice by modulating the composition of the gut microbiota and metabolites. The expression of genes and proteins in the PI3K/AKT pathway was stimulated by B. longum subsp. infantis CCFM1269 through the GH/IGF-1 axis in growing mice. This finding suggests B. longum subsp. infantis CCFM1269 may be useful for modulating bone metabolism during growth.

Published in Gut Microbes

ISSN: 1949-0976 (Print); 1949-0984 (Online)
Publisher: Taylor & Francis Group
Country of publisher: United States
LCC subjects: Medicine: Internal medicine: Specialties of internal medicine: Diseases of the digestive system. Gastroenterology
Website: https://www.tandfonline.com/journals/kgmi

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