MOLECULAR GENOTYPING OF RUMEN FUNGI BASED ON RFLP ANALYSIS

Abstract

Eight polycentric strains of the genera Orpinomyces and five monocentric strains of the genera Neocallimastix were examined for the restriction fragment length polymorphisms. EcoRI-digested nuclear DNA of isolates of rumen fungi and DNA-DNA hybridization with probe RS2 revealed several distinct cleavage profiles. Results are ambiguous and interpretation is difficult with respect to the high genetic variability of rumen fungi isolates.

Keywords

• microbiology
• anaerobic fungi
• molecular genetics
• RFLP
• classification
• rumen