PLoS ONE (Mar 2010)

The complete genome sequence of Haloferax volcanii DS2, a model archaeon.

  • Amber L Hartman,
  • Cédric Norais,
  • Jonathan H Badger,
  • Stéphane Delmas,
  • Sam Haldenby,
  • Ramana Madupu,
  • Jeffrey Robinson,
  • Hoda Khouri,
  • Qinghu Ren,
  • Todd M Lowe,
  • Julie Maupin-Furlow,
  • Mecky Pohlschroder,
  • Charles Daniels,
  • Friedhelm Pfeiffer,
  • Thorsten Allers,
  • Jonathan A Eisen

DOI
https://doi.org/10.1371/journal.pone.0009605
Journal volume & issue
Vol. 5, no. 3
p. e9605

Abstract

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Haloferax volcanii is an easily culturable moderate halophile that grows on simple defined media, is readily transformable, and has a relatively stable genome. This, in combination with its biochemical and genetic tractability, has made Hfx. volcanii a key model organism, not only for the study of halophilicity, but also for archaeal biology in general.We report here the sequencing and analysis of the genome of Hfx. volcanii DS2, the type strain of this species. The genome contains a main 2.848 Mb chromosome, three smaller chromosomes pHV1, 3, 4 (85, 438, 636 kb, respectively) and the pHV2 plasmid (6.4 kb).The completed genome sequence, presented here, provides an invaluable tool for further in vivo and in vitro studies of Hfx. volcanii.