Ap4A Regulates Directional Mobility and Antigen Presentation in Dendritic Cells

Shin La Shu; Lakshmi Bhargavi Paruchuru; Neil Quanwei Tay; Yen Leong Chua; Adeline Shen Yun Foo; Chris Maolin Yang; Ka Hang Liong; Esther Geok Liang Koh; Angeline Lee; Hovav Nechushtan; Ehud Razin; David Michael Kemeny

iScience (Jun 2019)

Ap4A Regulates Directional Mobility and Antigen Presentation in Dendritic Cells

Shin La Shu,
Lakshmi Bhargavi Paruchuru,
Neil Quanwei Tay,
Yen Leong Chua,
Adeline Shen Yun Foo,
Chris Maolin Yang,
Ka Hang Liong,
Esther Geok Liang Koh,
Angeline Lee,
Hovav Nechushtan,
Ehud Razin,
David Michael Kemeny

Affiliations

Shin La Shu: Immunology Programme, Department of Microbiology, Centre for Life Sciences, National University of Singapore, #03-09, 28 Medical Drive, Singapore 117456, Singapore; NUS-HUJ-CREATE, 1 Create Way, Innovation Wing #03-09, Singapore 138602, Singapore
Lakshmi Bhargavi Paruchuru: Department of Biochemistry and Molecular Biology, Institute for Medical Research Israel-Canada, Hebrew University of Jerusalem, Jerusalem 91120, Israel
Neil Quanwei Tay: Immunology Programme, Department of Microbiology, Centre for Life Sciences, National University of Singapore, #03-09, 28 Medical Drive, Singapore 117456, Singapore; NUS-HUJ-CREATE, 1 Create Way, Innovation Wing #03-09, Singapore 138602, Singapore
Yen Leong Chua: Immunology Programme, Department of Microbiology, Centre for Life Sciences, National University of Singapore, #03-09, 28 Medical Drive, Singapore 117456, Singapore
Adeline Shen Yun Foo: NUS-HUJ-CREATE, 1 Create Way, Innovation Wing #03-09, Singapore 138602, Singapore
Chris Maolin Yang: Immunology Programme, Department of Microbiology, Centre for Life Sciences, National University of Singapore, #03-09, 28 Medical Drive, Singapore 117456, Singapore; NUS-HUJ-CREATE, 1 Create Way, Innovation Wing #03-09, Singapore 138602, Singapore
Ka Hang Liong: Immunology Programme, Department of Microbiology, Centre for Life Sciences, National University of Singapore, #03-09, 28 Medical Drive, Singapore 117456, Singapore; NUS-HUJ-CREATE, 1 Create Way, Innovation Wing #03-09, Singapore 138602, Singapore
Esther Geok Liang Koh: Immunology Programme, Department of Microbiology, Centre for Life Sciences, National University of Singapore, #03-09, 28 Medical Drive, Singapore 117456, Singapore; NUS-HUJ-CREATE, 1 Create Way, Innovation Wing #03-09, Singapore 138602, Singapore
Angeline Lee: Immunology Programme, Department of Microbiology, Centre for Life Sciences, National University of Singapore, #03-09, 28 Medical Drive, Singapore 117456, Singapore; NUS-HUJ-CREATE, 1 Create Way, Innovation Wing #03-09, Singapore 138602, Singapore
Hovav Nechushtan: Sharett Institute of Oncology, Hadassah Hebrew University Medical Center, Hebrew University of Jerusalem, POB 12000 Jerusalem, Israel; Corresponding author
Ehud Razin: NUS-HUJ-CREATE, 1 Create Way, Innovation Wing #03-09, Singapore 138602, Singapore; Department of Biochemistry and Molecular Biology, Institute for Medical Research Israel-Canada, Hebrew University of Jerusalem, Jerusalem 91120, Israel; Corresponding author
David Michael Kemeny: Immunology Programme, Department of Microbiology, Centre for Life Sciences, National University of Singapore, #03-09, 28 Medical Drive, Singapore 117456, Singapore; NUS-HUJ-CREATE, 1 Create Way, Innovation Wing #03-09, Singapore 138602, Singapore; Corresponding author

Journal volume & issue: Vol. 16
pp. 524 – 534

Abstract

Read online

Summary: The significance of intracellular Ap4A levels over immune activity of dendritic cells (DCs) has been studied in Nudt2fl/fl/CD11c-cre mice. The transgenic mice have been generated by crossing floxed NUDT2 gene mice with DC marker CD11c recombinase (cre) mice. The DCs derived from these mice have higher levels of Ap4A (≈30-fold) compared with those derived from Nudt2+/+ mice. Interestingly, the elevated Ap4A in DCs has led them to possess higher motility and lower directional variability. In addition, the DCs are able to enhance immune protection indicated by the higher cross-presentation of antigen and priming of CD8+ OT-I T cells. Overall, the study denotes prominent impact of Ap4A over the functionality of DCs. The Nudt2fl/fl/CD11c-cre mice could serve as a useful tool to study the influence of Ap4A in the critical immune mechanisms of DCs. : Molecular Mechanism of Behavior; Immunology; Immune Response; Model Organism Subject Areas: Molecular Mechanism of Behavior, Immunology, Immune Response, Model Organism

Published in iScience

ISSN: 2589-0042 (Online)
Publisher: Elsevier
Country of publisher: United States
LCC subjects: Science
Website: http://www.cell.com/iscience/home

About the journal