Acta Cirúrgica Brasileira (Dec 2019)

The liver injury following ischemia and reperfusion is worse in experimental knockout heterozygote mouse model for expression of connexin 43

  • Alexandre Maximiliano Trevisan,
  • Bruno Cogliati,
  • Adriana Ribeiro Homem,
  • Thiago Pinheiro Arrais Aloiav,
  • Nelson de Aquino Neto,
  • Jairo Marques Moreira,
  • Leonardo da Cruz Reno,
  • Alexandre Moulin Naumann,
  • Flavio Henrique Ferreira Galvão,
  • Wellington Andraus,
  • Luiz Augusto Carneiro D'Albuquerque

DOI
https://doi.org/10.1590/s0102-865020190100000003
Journal volume & issue
Vol. 34, no. 10

Abstract

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Abstract Purpose: To evaluate that Connexin (Cx43) plays a role in lesions after hepatic ischemia/reperfusion (IR) injury. Methods: We use Cx43 deficient model (heterozygotes mice) and compared to a wild group. The groups underwent 1 hour ischemia and 24 hours reperfusion. The heterozygote genotype was confirmed by PCR. We analyzed the hepatic enzymes (AST, ALT, GGT) and histology. Results: The mice with Cx43 deficiency showed an ALT mean value of 4166 vs. 307 in the control group (p<0.001); AST mean value of 7231 vs. 471 in the control group (p<0.001); GGT mean value of 9.4 vs. 1.7 in the control group (p=0.001); histology showed necrosis and inflammation in the knockout group. Conclusions: This research demonstrated that the deficiency of Cx43 worses the prognosis for liver injury. The topic is a promising target for therapeutics advancements in liver diseases and procedures.

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