Ciência Animal Brasileira (Jun 2011)

AVALIAÇÃO MORFOLÓGICA DE CÓRNEAS DE COELHOS CRIOPRESERVADAS COM ETILENOGLICOL

  • LORENA BORGES ALVES,
  • DUVALDO EURIDES,
  • LILIAN VIEIRA DE ANDRADE,
  • LUIZ ANTÔNIO FRANCO DA FRANCO DA SILVA,
  • BENITO JUAREZ NUNES ALVES DE OLIVEIRA,
  • LUIZ AUGUSTO DE SOUZA

Journal volume & issue
Vol. 12, no. 2
pp. 376 – 381

Abstract

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Rabbit corneas, cryopreserved with ethylene glycol, were evaluated. Corneas of the right and left ocular globes were collected from eleven New Zelanda male rabbits, weighing between 4.0kg and 5.0kg, immediately after the animals were slaughtered. The corneas were removed with a 2.0mm edge of sclera and washed in physiological solution 0.9%. The right corneas were fixed in formaldeide, enclosed in paraffin, stained in hematoxyline eosine for evaluation in light microscopy. The left corneas were immersed in cryoprotect bottlescontaining phosphate-buffered saline solution, 0.4% of bovine albumen and 1.5 molar of ethylene glycol. The bottles were frozen for 60 days at -196º C in liquid nitrogen. After thawing, they were submitted to macrocospic analysis, and measured from digital images. The rabbits´ corneas preservated with ethylene glycol in liquid nitrogen caused no structural nor morphometry alterations at the layers that compose the cornea.

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