Nature Communications (Jan 2019)

C1 CAGE detects transcription start sites and enhancer activity at single-cell resolution

  • Tsukasa Kouno,
  • Jonathan Moody,
  • Andrew Tae-Jun Kwon,
  • Youtaro Shibayama,
  • Sachi Kato,
  • Yi Huang,
  • Michael Böttcher,
  • Efthymios Motakis,
  • Mickaël Mendez,
  • Jessica Severin,
  • Joachim Luginbühl,
  • Imad Abugessaisa,
  • Akira Hasegawa,
  • Satoshi Takizawa,
  • Takahiro Arakawa,
  • Masaaki Furuno,
  • Naveen Ramalingam,
  • Jay West,
  • Harukazu Suzuki,
  • Takeya Kasukawa,
  • Timo Lassmann,
  • Chung-Chau Hon,
  • Erik Arner,
  • Piero Carninci,
  • Charles Plessy,
  • Jay W. Shin

DOI
https://doi.org/10.1038/s41467-018-08126-5
Journal volume & issue
Vol. 10, no. 1
pp. 1 – 12

Abstract

Read online

Single-cell transcriptomic profiling allows the exploration of cellular heterogeneity but commonly focuses on the 3′-end of the transcript. Here the authors introduce C1 CAGE, which detects the 5′ transcript end in a multiplexed microfluidic system.