低共熔溶剂提取油茶籽壳中原花青素的工艺优化Optimization of extraction of procyanidins from oil-tea camellia seed shell with deep eutectic solvents

Abstract

为促进油茶籽副产物的高值化利用，采用低共熔溶剂提取油茶籽壳中的原花青素，通过单因素试验考察了提取溶剂种类、摄取溶剂质量分数、料液比、提取温度和提取时间对原花青素得率的影响，并利用正交试验对油茶籽壳中原花青素提取的工艺条件进行优化。结果表明：低共熔溶剂提取油茶籽壳中原花青素的最优工艺条件为以氯化胆碱-柠檬酸（物质的量比1∶ 1）溶液为提取溶剂、氯化胆碱-柠檬酸溶液质量分数80%、料液比1∶ 25、提取温度80 ℃、提取时间40 min，在此条件下原花青素得率为5.26%。综上，低共熔溶剂可有效提取油茶籽壳中的原花青素。 To promote the high value utilization of by-products from oil-tea camellia seed, procyanidins was extracted from oil-tea camellia seed shell with deep eutectic solvents，the effects of extraction solvent type, mass fraction of solvent, solid-liquid ratio, extraction temperature and extraction time on the yield of procyanidins were investigated by single factor experiment and process conditions for extraction of procyanidins from oil-tea camellia seed shell were optimized by orthogonal experiment. The results showed that the optimal extraction conditions were obtained as follows: with choline chloride-citric acid (molar ratio 1∶ 1) solution as extraction solvent, mass fraction of choline chloride-citric acid solution 80%, solid-liquid ratio 1∶ 25, extraction temperature 80 ℃, extraction time 40 min. Under these conditions, the yield of procyanidins was 5.26%.In conclusion, the procyanidins can be effectively extracted from oil-tea camellia seed shell with deep eutectic solvents.

Keywords

• 低共熔溶剂；提取；油茶籽壳；原花青素；正交优化
• deep eutectic solvents; extraction; oil-tea camellia seed shell; procyanidins; orthogonal optimization