Widespread intronic polyadenylation diversifies immune cell transcriptomes

Irtisha Singh; Shih-Han Lee; Adam S. Sperling; Mehmet K. Samur; Yu-Tzu Tai; Mariateresa Fulciniti; Nikhil C. Munshi; Christine Mayr; Christina S. Leslie

doi:10.1038/s41467-018-04112-z

Nature Communications (Apr 2018)

Widespread intronic polyadenylation diversifies immune cell transcriptomes

Irtisha Singh,
Shih-Han Lee,
Adam S. Sperling,
Mehmet K. Samur,
Yu-Tzu Tai,
Mariateresa Fulciniti,
Nikhil C. Munshi,
Christine Mayr,
Christina S. Leslie

Affiliations

Irtisha Singh: Computational and Systems Biology Program, Memorial Sloan Kettering Cancer Center
Shih-Han Lee: Cancer Biology and Genetics Program, Memorial Sloan Kettering Cancer Center
Adam S. Sperling: Lebow Institute of Myeloma Therapeutics and Jerome Lipper Multiple Myeloma Center, Dana-Farber Cancer Institute, Harvard Medical School
Mehmet K. Samur: Lebow Institute of Myeloma Therapeutics and Jerome Lipper Multiple Myeloma Center, Dana-Farber Cancer Institute, Harvard Medical School
Yu-Tzu Tai: Lebow Institute of Myeloma Therapeutics and Jerome Lipper Multiple Myeloma Center, Dana-Farber Cancer Institute, Harvard Medical School
Mariateresa Fulciniti: Lebow Institute of Myeloma Therapeutics and Jerome Lipper Multiple Myeloma Center, Dana-Farber Cancer Institute, Harvard Medical School
Nikhil C. Munshi: Lebow Institute of Myeloma Therapeutics and Jerome Lipper Multiple Myeloma Center, Dana-Farber Cancer Institute, Harvard Medical School
Christine Mayr: Cancer Biology and Genetics Program, Memorial Sloan Kettering Cancer Center
Christina S. Leslie: Computational and Systems Biology Program, Memorial Sloan Kettering Cancer Center

DOI: https://doi.org/10.1038/s41467-018-04112-z
Journal volume & issue: Vol. 9, no. 1
pp. 1 – 16

Abstract

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Recognition of intronic polyadenylation (IpA) signals can lead to expression of truncated proteins lacking C terminal domains. Analysis of 3ʹ -seq and RNA-seq shows that IpA is widespread in circulating immune cells, while multiple myeloma cells show loss of IpA isoforms that are normally expressed in plasma cells, impacting key genes in the disease.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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