Optical tweezers-controlled hotspot for sensitive and reproducible surface-enhanced Raman spectroscopy characterization of native protein structures

Xin Dai; Wenhao Fu; Huanyu Chi; Vince St. Dollente Mesias; Hongni Zhu; Cheuk Wai Leung; Wei Liu; Jinqing Huang

doi:10.1038/s41467-021-21543-3

Nature Communications (Feb 2021)

Optical tweezers-controlled hotspot for sensitive and reproducible surface-enhanced Raman spectroscopy characterization of native protein structures

Xin Dai,
Wenhao Fu,
Huanyu Chi,
Vince St. Dollente Mesias,
Hongni Zhu,
Cheuk Wai Leung,
Wei Liu,
Jinqing Huang

Affiliations

Xin Dai: Department of Chemistry, The Hong Kong University of Science and Technology
Wenhao Fu: Department of Chemistry, The Hong Kong University of Science and Technology
Huanyu Chi: Department of Chemistry, The Hong Kong University of Science and Technology
Vince St. Dollente Mesias: Department of Chemistry, The Hong Kong University of Science and Technology
Hongni Zhu: Department of Chemistry, The Hong Kong University of Science and Technology
Cheuk Wai Leung: Department of Chemistry, The Hong Kong University of Science and Technology
Wei Liu: State Key Laboratory of Synthetic Chemistry, Department of Chemistry, The University of Hong Kong
Jinqing Huang: Department of Chemistry, The Hong Kong University of Science and Technology

DOI: https://doi.org/10.1038/s41467-021-21543-3
Journal volume & issue: Vol. 12, no. 1
pp. 1 – 9

Abstract

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It is currently challenging to identify protein structures at low concentrations. Here the authors report optical tweezers-coupled Raman spectroscopy to generate tunable and reproducible SERS enhancements with single-molecule level sensitivity and use the method to detect protein structural features.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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