Molecules (Oct 2019)
Spider Chitin: An Ultrafast Microwave-Assisted Method for Chitin Isolation from <i>Caribena versicolor</i> Spider Molt Cuticle
- Tomasz Machałowski,
- Marcin Wysokowski,
- Mikhail V. Tsurkan,
- Roberta Galli,
- Christian Schimpf,
- David Rafaja,
- Erica Brendler,
- Christine Viehweger,
- Sonia Żółtowska-Aksamitowska,
- Iaroslav Petrenko,
- Katarzyna Czaczyk,
- Michael Kraft,
- Martin Bertau,
- Nicole Bechmann,
- Kaomei Guan,
- Stefan R. Bornstein,
- Alona Voronkina,
- Andriy Fursov,
- Magdalena Bejger,
- Katarzyna Biniek-Antosiak,
- Wojciech Rypniewski,
- Marek Figlerowicz,
- Oleg Pokrovsky,
- Teofil Jesionowski,
- Hermann Ehrlich
Affiliations
- Tomasz Machałowski
- Institute of Chemical Technology and Engineering, Faculty of Chemical Technology, Poznan University of Technology, 60965 Poznan, Poland
- Marcin Wysokowski
- Institute of Chemical Technology and Engineering, Faculty of Chemical Technology, Poznan University of Technology, 60965 Poznan, Poland
- Mikhail V. Tsurkan
- Leibniz Institute of Polymer Research Dresden, Dresden 01069, Germany
- Roberta Galli
- Clinical Sensoring and Monitoring, Department of Anesthesiology and Intensive Care Medicine, Faculty of Medicine, TU Dresden, 01307 Dresden, Germany
- Christian Schimpf
- Institute of Materials Science, TU Bergakademie Freiberg, 09599 Freiberg, Germany
- David Rafaja
- Institute of Materials Science, TU Bergakademie Freiberg, 09599 Freiberg, Germany
- Erica Brendler
- Institute of Analytical Chemistry, TU Bergakademie Freiberg, 09599 Freiberg, Germany
- Christine Viehweger
- Institute of Analytical Chemistry, TU Bergakademie Freiberg, 09599 Freiberg, Germany
- Sonia Żółtowska-Aksamitowska
- Institute of Chemical Technology and Engineering, Faculty of Chemical Technology, Poznan University of Technology, 60965 Poznan, Poland
- Iaroslav Petrenko
- Institute of Electronics and Sensor Materials, TU Bergakademie Freiberg, 09599 Freiberg, Germany
- Katarzyna Czaczyk
- Department of Biotechnology and Food Microbiology, Poznan University of Life Sciences, 60637 Poznan, Poland
- Michael Kraft
- Institute of Chemical Technology, TU Bergakademie Freiberg, 09599 Freiberg, Germany
- Martin Bertau
- Institute of Chemical Technology, TU Bergakademie Freiberg, 09599 Freiberg, Germany
- Nicole Bechmann
- Institute of Clinical Chemistry and Laboratory Medicine, University Hospital Carl Gustav Carus, TU Dresden, 01307 Dresden, Germany
- Kaomei Guan
- Institute of Pharmacology and Toxicology, TU Dresden, 01307 Dresden, Germany
- Stefan R. Bornstein
- Center for Regenerative Therapies Dresden, TU Dresden, 01307 Dresden, Germany
- Alona Voronkina
- Department of Pharmacy, National Pirogov Memorial Medical University, 21018 Vinnytsia, Ukraine
- Andriy Fursov
- Institute of Electronics and Sensor Materials, TU Bergakademie Freiberg, 09599 Freiberg, Germany
- Magdalena Bejger
- Institute of Bioorganic Chemistry, Polish Academy of Sciences, 61704 Poznan, Poland
- Katarzyna Biniek-Antosiak
- Institute of Bioorganic Chemistry, Polish Academy of Sciences, 61704 Poznan, Poland
- Wojciech Rypniewski
- Institute of Bioorganic Chemistry, Polish Academy of Sciences, 61704 Poznan, Poland
- Marek Figlerowicz
- Institute of Bioorganic Chemistry, Polish Academy of Sciences, 61704 Poznan, Poland
- Oleg Pokrovsky
- Geoscience and Environment Toulouse, UMR 5563 CNRS, 31400 Toulouse, France
- Teofil Jesionowski
- Institute of Chemical Technology and Engineering, Faculty of Chemical Technology, Poznan University of Technology, 60965 Poznan, Poland
- Hermann Ehrlich
- Institute of Electronics and Sensor Materials, TU Bergakademie Freiberg, 09599 Freiberg, Germany
- DOI
- https://doi.org/10.3390/molecules24203736
- Journal volume & issue
-
Vol. 24,
no. 20
p. 3736
Abstract
Chitin, as a fundamental polysaccharide in invertebrate skeletons, continues to be actively investigated, especially with respect to new sources and the development of effective methods for its extraction. Recent attention has been focused on marine crustaceans and sponges; however, the potential of spiders (order Araneae) as an alternative source of tubular chitin has been overlooked. In this work, we focused our attention on chitin from up to 12 cm-large Theraphosidae spiders, popularly known as tarantulas or bird-eating spiders. These organisms “lose” large quantities of cuticles during their molting cycle. Here, we present for the first time a highly effective method for the isolation of chitin from Caribena versicolor spider molt cuticle, as well as its identification and characterization using modern analytical methods. We suggest that the tube-like molt cuticle of this spider can serve as a naturally prefabricated and renewable source of tubular chitin with high potential for application in technology and biomedicine.
Keywords
