Tissue alkalosis in cold-ischemia time

V. Denninghoff; E. H. R. Olivieri; C. Fresno; A. Uceda; L. Mota; A. P. Suenaga; D. M. Carraro; V. R. Martins; A. Avagnina; F. A. Soares; A. H. J. Fróes Marques Campos

doi:10.1038/s41598-017-11284-z

Scientific Reports (Sep 2017)

Tissue alkalosis in cold-ischemia time

V. Denninghoff,
E. H. R. Olivieri,
C. Fresno,
A. Uceda,
L. Mota,
A. P. Suenaga,
D. M. Carraro,
V. R. Martins,
A. Avagnina,
F. A. Soares,
A. H. J. Fróes Marques Campos

Affiliations

V. Denninghoff: Department of Pathology, A. C. Camargo Cancer Center
E. H. R. Olivieri: International Center for Research (CIPE), A. C. Camargo Cancer Center
C. Fresno: Computational Genomics Division, National Institute of Genomic Medicine (INMEGEN)
A. Uceda: Department of Pathology & Biobank, Center for Medical Education and Clinical Research “Norberto Quirno” (CEMIC), Ciudad Autónoma de Buenos Aires
L. Mota: A. C. Camargo Biobank, A. C. Camargo Cancer Center
A. P. Suenaga: A. C. Camargo Biobank, A. C. Camargo Cancer Center
D. M. Carraro: International Center for Research (CIPE), A. C. Camargo Cancer Center
V. R. Martins: International Center for Research (CIPE), A. C. Camargo Cancer Center
A. Avagnina: Department of Pathology & Biobank, Center for Medical Education and Clinical Research “Norberto Quirno” (CEMIC), Ciudad Autónoma de Buenos Aires
F. A. Soares: Department of Pathology, A. C. Camargo Cancer Center
A. H. J. Fróes Marques Campos: Department of Pathology, A. C. Camargo Cancer Center

DOI: https://doi.org/10.1038/s41598-017-11284-z
Journal volume & issue: Vol. 7, no. 1
pp. 1 – 6

Abstract

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Abstract The control of pre-analytical-factors in human biospecimens collected for health research is currently required. Only two previous reports using post-mortem brain samples have tried to address the impact of cold-ischemia on tissue pH. Here we report pH variations according to time (third-order polynomial model) in mice for liver, kidney and lung samples. Tissue alkalosis in cold-ischemia time may be an underlying mechanism of gene expression changes. Therefore, tissue-pH regulation after organ removal may minimize biological stress in human tissue samples.

Published in Scientific Reports

ISSN: 2045-2322 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Medicine; Science
Website: https://www.nature.com/srep/

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