Study of the influence of disintegrated biomass of chlorella sorokiniana on the state of yeast saccharomyces cerevisiae

Abstract

The physiological state of Saccharomyces cerevisiae yeast used in fermentation plants is strongly influenced by various stress factors, “stimulants” and top dressing. Effective yeast activation reduces the time it takes to prepare the finished product and increase its quality. Chlorella sorokiniana is a fast-growing microalgae, its biomass is a promising source of protein, carbohydrates, antioxidants, vitamins, magnesium and other components. Its cells have a powerful cell membrane, so the necessary step is the disintegration of the microalgae biomass. Ultrasonic, microwave disintegration methods, as well as mechanical using a high-speed homogenizer were used. When introducing disintegrated biomass at a concentration of 20 mg%, the most effective option compared to the control (without additives) was the use of microwave disintegration: an increase in ethanol-forming ability (by 43.5%), a visible degree of fermentation (by 39.2%), and yeast concentration cells in the population (by 31.0%). Ultrasonic and mechanical disintegration of microalgae biomass is less effective. Thus, the use of Chlorella sorokiniana microalgae as a source of valuable components is a promising way of activating the Saccharomyces cerevisiae yeast, which requires additional research in the development of technology for preliminary disintegration and complex processing of introduced biomass.