Dynamic Runx1 chromatin boundaries affect gene expression in hematopoietic development

Dominic D. G. Owens; Giorgio Anselmi; A. Marieke Oudelaar; Damien J. Downes; Alessandro Cavallo; Joe R. Harman; Ron Schwessinger; Akin Bucakci; Lucas Greder; Sara de Ornellas; Danuta Jeziorska; Jelena Telenius; Jim R. Hughes; Marella F. T. R. de Bruijn

doi:10.1038/s41467-022-28376-8

Nature Communications (Feb 2022)

Dynamic Runx1 chromatin boundaries affect gene expression in hematopoietic development

Dominic D. G. Owens,
Giorgio Anselmi,
A. Marieke Oudelaar,
Damien J. Downes,
Alessandro Cavallo,
Joe R. Harman,
Ron Schwessinger,
Akin Bucakci,
Lucas Greder,
Sara de Ornellas,
Danuta Jeziorska,
Jelena Telenius,
Jim R. Hughes,
Marella F. T. R. de Bruijn

Affiliations

Dominic D. G. Owens: MRC Molecular Hematology Unit, MRC Weatherall Institute of Molecular Medicine, Radcliffe Department of Medicine, University of Oxford
Giorgio Anselmi: MRC Molecular Hematology Unit, MRC Weatherall Institute of Molecular Medicine, Radcliffe Department of Medicine, University of Oxford
A. Marieke Oudelaar: MRC Molecular Hematology Unit, MRC Weatherall Institute of Molecular Medicine, Radcliffe Department of Medicine, University of Oxford
Damien J. Downes: MRC Molecular Hematology Unit, MRC Weatherall Institute of Molecular Medicine, Radcliffe Department of Medicine, University of Oxford
Alessandro Cavallo: MRC Molecular Hematology Unit, MRC Weatherall Institute of Molecular Medicine, Radcliffe Department of Medicine, University of Oxford
Joe R. Harman: MRC Molecular Hematology Unit, MRC Weatherall Institute of Molecular Medicine, Radcliffe Department of Medicine, University of Oxford
Ron Schwessinger: MRC Molecular Hematology Unit, MRC Weatherall Institute of Molecular Medicine, Radcliffe Department of Medicine, University of Oxford
Akin Bucakci: MRC Molecular Hematology Unit, MRC Weatherall Institute of Molecular Medicine, Radcliffe Department of Medicine, University of Oxford
Lucas Greder: MRC Molecular Hematology Unit, MRC Weatherall Institute of Molecular Medicine, Radcliffe Department of Medicine, University of Oxford
Sara de Ornellas: MRC Molecular Hematology Unit, MRC Weatherall Institute of Molecular Medicine, Radcliffe Department of Medicine, University of Oxford
Danuta Jeziorska: MRC Molecular Hematology Unit, MRC Weatherall Institute of Molecular Medicine, Radcliffe Department of Medicine, University of Oxford
Jelena Telenius: MRC WIMM Centre for Computational Biology, MRC Weatherall Institute of Molecular Medicine, Radcliffe Department of Medicine, University of Oxford
Jim R. Hughes: MRC Molecular Hematology Unit, MRC Weatherall Institute of Molecular Medicine, Radcliffe Department of Medicine, University of Oxford
Marella F. T. R. de Bruijn: MRC Molecular Hematology Unit, MRC Weatherall Institute of Molecular Medicine, Radcliffe Department of Medicine, University of Oxford

DOI: https://doi.org/10.1038/s41467-022-28376-8
Journal volume & issue: Vol. 13, no. 1
pp. 1 – 15

Abstract

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RUNX1 is a large and complex gene with two alternative promoters and multiple hematopoietic enhancers. Here the authors show that unlike smaller genes Runx1 becomes sub-compartmentalized during differentiation and gene activation. This subcompartmentalization partially depends on CTCF binding at promoter-proximal CTCF sites and transcription.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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