Profiling the responsiveness of focal adhesions of human cardiomyocytes to extracellular dynamic nano-topography

Huaiyu Shi; Xiangjun Wu; Shiyang Sun; Chenyan Wang; Zacharias Vangelatos; Ariel Ash-Shakoor; Costas P. Grigoropoulos; Patrick T. Mather; James H. Henderson; Zhen Ma

Bioactive Materials (Apr 2022)

Profiling the responsiveness of focal adhesions of human cardiomyocytes to extracellular dynamic nano-topography

Huaiyu Shi,
Xiangjun Wu,
Shiyang Sun,
Chenyan Wang,
Zacharias Vangelatos,
Ariel Ash-Shakoor,
Costas P. Grigoropoulos,
Patrick T. Mather,
James H. Henderson,
Zhen Ma

Affiliations

Huaiyu Shi: Department of Biomedical & Chemical Engineering, Syracuse University, Syracuse, NY, 13244, USA; BioInspired Syracuse Institute for Materials and Living Systems, Syracuse University, Syracuse, NY, 13244, USA
Xiangjun Wu: Department of Biomedical & Chemical Engineering, Syracuse University, Syracuse, NY, 13244, USA; BioInspired Syracuse Institute for Materials and Living Systems, Syracuse University, Syracuse, NY, 13244, USA
Shiyang Sun: Department of Biomedical & Chemical Engineering, Syracuse University, Syracuse, NY, 13244, USA; BioInspired Syracuse Institute for Materials and Living Systems, Syracuse University, Syracuse, NY, 13244, USA
Chenyan Wang: Department of Biomedical & Chemical Engineering, Syracuse University, Syracuse, NY, 13244, USA; BioInspired Syracuse Institute for Materials and Living Systems, Syracuse University, Syracuse, NY, 13244, USA
Zacharias Vangelatos: Department of Mechanical Engineering, University of California, Berkeley, PA, 94720, USA
Ariel Ash-Shakoor: Department of Biomedical & Chemical Engineering, Syracuse University, Syracuse, NY, 13244, USA
Costas P. Grigoropoulos: Department of Mechanical Engineering, University of California, Berkeley, PA, 94720, USA
Patrick T. Mather: Department of Chemical Engineering, Bucknell University, Lewisburg, PA, 17837, USA
James H. Henderson: Department of Biomedical & Chemical Engineering, Syracuse University, Syracuse, NY, 13244, USA; BioInspired Syracuse Institute for Materials and Living Systems, Syracuse University, Syracuse, NY, 13244, USA
Zhen Ma: Department of Biomedical & Chemical Engineering, Syracuse University, Syracuse, NY, 13244, USA; BioInspired Syracuse Institute for Materials and Living Systems, Syracuse University, Syracuse, NY, 13244, USA; Corresponding author. Department of Biomedical & Chemical Engineering, Syracuse University, Syracuse, NY, 13244, USA.

Journal volume & issue: Vol. 10
pp. 367 – 377

Abstract

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Focal adhesion complexes function as the mediators of cell-extracellular matrix interactions to sense and transmit the extracellular signals. Previous studies have demonstrated that cardiomyocyte focal adhesions can be modulated by surface topographic features. However, the response of focal adhesions to dynamic surface topographic changes remains underexplored. To study this dynamic responsiveness of focal adhesions, we utilized a shape memory polymer-based substrate that can produce a flat-to-wrinkle surface transition triggered by an increase of temperature. Using this dynamic culture system, we analyzed three proteins (paxillin, vinculin and zyxin) from different layers of the focal adhesion complex in response to dynamic extracellular topographic change. Hence, we quantified the dynamic profile of cardiomyocyte focal adhesion in a time-dependent manner, which provides new understanding of dynamic cardiac mechanobiology.

Published in Bioactive Materials

ISSN: 2452-199X (Online)
Publisher: KeAi Communications Co., Ltd.
Country of publisher: China
LCC subjects: Technology: Electrical engineering. Electronics. Nuclear engineering: Materials of engineering and construction. Mechanics of materials; Science: Biology (General)
Website: https://www.keaipublishing.com/en/journals/bioactive-materials/

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