Cell Reports (Aug 2023)
Histone H1 protects telomeric repeats from H3K27me3 invasion in Arabidopsis
- Gianluca Teano,
- Lorenzo Concia,
- Léa Wolff,
- Léopold Carron,
- Ivona Biocanin,
- Kateřina Adamusová,
- Miloslava Fojtová,
- Michael Bourge,
- Amira Kramdi,
- Vincent Colot,
- Ueli Grossniklaus,
- Chris Bowler,
- Célia Baroux,
- Alessandra Carbone,
- Aline V. Probst,
- Petra Procházková Schrumpfová,
- Jiří Fajkus,
- Simon Amiard,
- Stefan Grob,
- Clara Bourbousse,
- Fredy Barneche
Affiliations
- Gianluca Teano
- Institut de biologie de l’École normale supérieure (IBENS), École normale supérieure, CNRS, INSERM, Université PSL, Paris, France; Université Paris-Saclay, 91190 Orsay, France
- Lorenzo Concia
- Institut de biologie de l’École normale supérieure (IBENS), École normale supérieure, CNRS, INSERM, Université PSL, Paris, France
- Léa Wolff
- Institut de biologie de l’École normale supérieure (IBENS), École normale supérieure, CNRS, INSERM, Université PSL, Paris, France
- Léopold Carron
- Sorbonne Université, CNRS, IBPS, UMR 7238, Laboratoire de Biologie Computationnelle et Quantitative (LCQB), 75005 Paris, France
- Ivona Biocanin
- Institut de biologie de l’École normale supérieure (IBENS), École normale supérieure, CNRS, INSERM, Université PSL, Paris, France; Université Paris-Saclay, 91190 Orsay, France
- Kateřina Adamusová
- Mendel Centre for Plant Genomics and Proteomics, Central European Institute of Technology, Masaryk University, Brno, Czech Republic; Laboratory of Functional Genomics and Proteomics, NCBR, Faculty of Science, Masaryk University, Brno, Czech Republic
- Miloslava Fojtová
- Mendel Centre for Plant Genomics and Proteomics, Central European Institute of Technology, Masaryk University, Brno, Czech Republic; Laboratory of Functional Genomics and Proteomics, NCBR, Faculty of Science, Masaryk University, Brno, Czech Republic
- Michael Bourge
- Cytometry Facility, Imagerie-Gif, Université Paris-Saclay, CEA, CNRS, Institute for Integrative Biology of the Cell (I2BC), 91198 Gif-sur-Yvette, France
- Amira Kramdi
- Institut de biologie de l’École normale supérieure (IBENS), École normale supérieure, CNRS, INSERM, Université PSL, Paris, France
- Vincent Colot
- Institut de biologie de l’École normale supérieure (IBENS), École normale supérieure, CNRS, INSERM, Université PSL, Paris, France
- Ueli Grossniklaus
- Department of Plant and Microbial Biology & Zurich-Basel Plant Science Center, University of Zurich, Zurich, Switzerland
- Chris Bowler
- Institut de biologie de l’École normale supérieure (IBENS), École normale supérieure, CNRS, INSERM, Université PSL, Paris, France
- Célia Baroux
- Department of Plant and Microbial Biology & Zurich-Basel Plant Science Center, University of Zurich, Zurich, Switzerland
- Alessandra Carbone
- Sorbonne Université, CNRS, IBPS, UMR 7238, Laboratoire de Biologie Computationnelle et Quantitative (LCQB), 75005 Paris, France
- Aline V. Probst
- CNRS UMR6293, Université Clermont Auvergne, INSERM U1103, GReD, CRBC, Clermont-Ferrand, France
- Petra Procházková Schrumpfová
- Mendel Centre for Plant Genomics and Proteomics, Central European Institute of Technology, Masaryk University, Brno, Czech Republic; Laboratory of Functional Genomics and Proteomics, NCBR, Faculty of Science, Masaryk University, Brno, Czech Republic
- Jiří Fajkus
- Mendel Centre for Plant Genomics and Proteomics, Central European Institute of Technology, Masaryk University, Brno, Czech Republic; Laboratory of Functional Genomics and Proteomics, NCBR, Faculty of Science, Masaryk University, Brno, Czech Republic
- Simon Amiard
- CNRS UMR6293, Université Clermont Auvergne, INSERM U1103, GReD, CRBC, Clermont-Ferrand, France
- Stefan Grob
- Department of Plant and Microbial Biology & Zurich-Basel Plant Science Center, University of Zurich, Zurich, Switzerland
- Clara Bourbousse
- Institut de biologie de l’École normale supérieure (IBENS), École normale supérieure, CNRS, INSERM, Université PSL, Paris, France
- Fredy Barneche
- Institut de biologie de l’École normale supérieure (IBENS), École normale supérieure, CNRS, INSERM, Université PSL, Paris, France; Corresponding author
- Journal volume & issue
-
Vol. 42,
no. 8
p. 112894
Abstract
Summary: While the pivotal role of linker histone H1 in shaping nucleosome organization is well established, its functional interplays with chromatin factors along the epigenome are just starting to emerge. Here we show that, in Arabidopsis, as in mammals, H1 occupies Polycomb Repressive Complex 2 (PRC2) target genes where it favors chromatin condensation and H3K27me3 deposition. We further show that, contrasting with its conserved function in PRC2 activation at genes, H1 selectively prevents H3K27me3 accumulation at telomeres and large pericentromeric interstitial telomeric repeat (ITR) domains by restricting DNA accessibility to Telomere Repeat Binding (TRB) proteins, a group of H1-related Myb factors mediating PRC2 cis recruitment. This study provides a mechanistic framework by which H1 avoids the formation of gigantic H3K27me3-rich domains at telomeric sequences and contributes to safeguard nucleus architecture.
