Nature Communications (Nov 2017)
Whole blood stabilization for the microfluidic isolation and molecular characterization of circulating tumor cells
- Keith H. K. Wong,
- Shannon N. Tessier,
- David T. Miyamoto,
- Kathleen L. Miller,
- Lauren D. Bookstaver,
- Thomas R. Carey,
- Cleo J. Stannard,
- Vishal Thapar,
- Eric C. Tai,
- Kevin D. Vo,
- Erin S. Emmons,
- Haley M. Pleskow,
- Rebecca D. Sandlin,
- Lecia V. Sequist,
- David T. Ting,
- Daniel A. Haber,
- Shyamala Maheswaran,
- Shannon L. Stott,
- Mehmet Toner
Affiliations
- Keith H. K. Wong
- BioMEMS Resource Center, Center for Engineering in Medicine, Massachusetts General Hospital, Harvard Medical School
- Shannon N. Tessier
- BioMEMS Resource Center, Center for Engineering in Medicine, Massachusetts General Hospital, Harvard Medical School
- David T. Miyamoto
- Cancer Center, Massachusetts General Hospital, Harvard Medical School
- Kathleen L. Miller
- BioMEMS Resource Center, Center for Engineering in Medicine, Massachusetts General Hospital, Harvard Medical School
- Lauren D. Bookstaver
- BioMEMS Resource Center, Center for Engineering in Medicine, Massachusetts General Hospital, Harvard Medical School
- Thomas R. Carey
- BioMEMS Resource Center, Center for Engineering in Medicine, Massachusetts General Hospital, Harvard Medical School
- Cleo J. Stannard
- BioMEMS Resource Center, Center for Engineering in Medicine, Massachusetts General Hospital, Harvard Medical School
- Vishal Thapar
- Cancer Center, Massachusetts General Hospital, Harvard Medical School
- Eric C. Tai
- Cancer Center, Massachusetts General Hospital, Harvard Medical School
- Kevin D. Vo
- Cancer Center, Massachusetts General Hospital, Harvard Medical School
- Erin S. Emmons
- Cancer Center, Massachusetts General Hospital, Harvard Medical School
- Haley M. Pleskow
- Department of Radiation Oncology, Massachusetts General Hospital, Harvard Medical School
- Rebecca D. Sandlin
- BioMEMS Resource Center, Center for Engineering in Medicine, Massachusetts General Hospital, Harvard Medical School
- Lecia V. Sequist
- Cancer Center, Massachusetts General Hospital, Harvard Medical School
- David T. Ting
- Cancer Center, Massachusetts General Hospital, Harvard Medical School
- Daniel A. Haber
- Cancer Center, Massachusetts General Hospital, Harvard Medical School
- Shyamala Maheswaran
- Department of Surgery, Massachusetts General Hospital, Harvard Medical School
- Shannon L. Stott
- BioMEMS Resource Center, Center for Engineering in Medicine, Massachusetts General Hospital, Harvard Medical School
- Mehmet Toner
- BioMEMS Resource Center, Center for Engineering in Medicine, Massachusetts General Hospital, Harvard Medical School
- DOI
- https://doi.org/10.1038/s41467-017-01705-y
- Journal volume & issue
-
Vol. 8,
no. 1
pp. 1 – 11
Abstract
The current FDA-approved whole blood stabilization method for circulating tumor cell (CTC) isolation suffers from RNA degradation. Here the authors combine hypothermic preservation and antiplatelet strategies to stabilize whole blood up to 72 h without compromising CTC yield and RNA integrity.
