Requisite Chromatin Remodeling for Myeloid and Erythroid Lineage Differentiation from Erythromyeloid Progenitors

Jun Wu; Karen Krchma; Hyung Joo Lee; Sairam Prabhakar; Xiaoli Wang; Haiyong Zhao; Xiaoyun Xing; Rho H. Seong; Daved H. Fremont; Maxim N. Artyomov; Ting Wang; Kyunghee Choi

Cell Reports (Nov 2020)

Requisite Chromatin Remodeling for Myeloid and Erythroid Lineage Differentiation from Erythromyeloid Progenitors

Jun Wu,
Karen Krchma,
Hyung Joo Lee,
Sairam Prabhakar,
Xiaoli Wang,
Haiyong Zhao,
Xiaoyun Xing,
Rho H. Seong,
Daved H. Fremont,
Maxim N. Artyomov,
Ting Wang,
Kyunghee Choi

Affiliations

Jun Wu: Department of Pathology and Immunology, Washington University School of Medicine, St. Louis, MO, USA
Karen Krchma: Department of Pathology and Immunology, Washington University School of Medicine, St. Louis, MO, USA
Hyung Joo Lee: Department of Genetics, Washington University School of Medicine, St. Louis, MO, USA; Edison Family Center for Genome Sciences and Systems Biology, Washington University School of Medicine, St. Louis, MO, USA
Sairam Prabhakar: Department of Pathology and Immunology, Washington University School of Medicine, St. Louis, MO, USA
Xiaoli Wang: Department of Pathology and Immunology, Washington University School of Medicine, St. Louis, MO, USA
Haiyong Zhao: Department of Pathology and Immunology, Washington University School of Medicine, St. Louis, MO, USA
Xiaoyun Xing: Department of Genetics, Washington University School of Medicine, St. Louis, MO, USA; Edison Family Center for Genome Sciences and Systems Biology, Washington University School of Medicine, St. Louis, MO, USA
Rho H. Seong: Department of Biological Sciences, Institute of Molecular Biology and Genetics, Seoul National University, Seoul, Korea
Daved H. Fremont: Department of Pathology and Immunology, Washington University School of Medicine, St. Louis, MO, USA
Maxim N. Artyomov: Department of Pathology and Immunology, Washington University School of Medicine, St. Louis, MO, USA
Ting Wang: Department of Genetics, Washington University School of Medicine, St. Louis, MO, USA; Edison Family Center for Genome Sciences and Systems Biology, Washington University School of Medicine, St. Louis, MO, USA; McDonnell Genome Institute, Washington University School of Medicine, St Louis, MO, USA
Kyunghee Choi: Department of Pathology and Immunology, Washington University School of Medicine, St. Louis, MO, USA; Graduate School of Biotechnology, Kyung Hee University, Yong In, Korea; Corresponding author

Journal volume & issue: Vol. 33, no. 7
p. 108395

Abstract

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Summary: The mammalian SWitch/Sucrose Non-Fermentable (SWI/SNF) chromatin-remodeling BAF (BRG1/BRM-associated factor) complex plays an essential role in developmental and pathological processes. We show that the deletion of Baf155, which encodes a subunit of the BAF complex, in the Tie2(+) lineage (Baf155 (CKO) leads to defects in yolk sac myeloid and definitive erythroid (EryD) lineage differentiation from erythromyeloid progenitors (EMPs). The chromatin of myeloid gene loci in Baf155 CKO EMPs is mostly inaccessible and enriched mainly by the ETS binding motif. BAF155 interacts with PU.1 and is recruited to PU.1 target gene loci together with p300 and KDM6a. Treatment of Baf155 CKO embryos with GSK126, an H3K27me2/3 methyltransferase EZH2 inhibitor, rescues myeloid lineage gene expression. This study uncovers indispensable BAF-mediated chromatin remodeling of myeloid gene loci at the EMP stage. Future studies exploiting epigenetics in the generation and application of EMP derivatives for tissue repair, regeneration, and disease are warranted.

Published in Cell Reports

ISSN: 2211-1247 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Biology (General)
Website: http://www.cell.com/cell-reports/home

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