Nature Communications (Sep 2019)
The cryo-EM structure of the acid activatable pore-forming immune effector Macrophage-expressed gene 1
- Siew Siew Pang,
- Charles Bayly-Jones,
- Mazdak Radjainia,
- Bradley A. Spicer,
- Ruby H. P. Law,
- Adrian W. Hodel,
- Edward S. Parsons,
- Susan M. Ekkel,
- Paul J. Conroy,
- Georg Ramm,
- Hariprasad Venugopal,
- Phillip I. Bird,
- Bart W. Hoogenboom,
- Ilia Voskoboinik,
- Yann Gambin,
- Emma Sierecki,
- Michelle A. Dunstone,
- James C. Whisstock
Affiliations
- Siew Siew Pang
- ARC Centre of Excellence in Advanced Molecular Imaging, Monash University
- Charles Bayly-Jones
- ARC Centre of Excellence in Advanced Molecular Imaging, Monash University
- Mazdak Radjainia
- Biomedicine Discovery Institute, Department of Biochemistry and Molecular Biology, Monash University
- Bradley A. Spicer
- ARC Centre of Excellence in Advanced Molecular Imaging, Monash University
- Ruby H. P. Law
- ARC Centre of Excellence in Advanced Molecular Imaging, Monash University
- Adrian W. Hodel
- London Centre for Nanotechnology, University College London
- Edward S. Parsons
- London Centre for Nanotechnology, University College London
- Susan M. Ekkel
- ARC Centre of Excellence in Advanced Molecular Imaging, Monash University
- Paul J. Conroy
- ARC Centre of Excellence in Advanced Molecular Imaging, Monash University
- Georg Ramm
- Biomedicine Discovery Institute, Department of Biochemistry and Molecular Biology, Monash University
- Hariprasad Venugopal
- Biomedicine Discovery Institute, Department of Biochemistry and Molecular Biology, Monash University
- Phillip I. Bird
- Biomedicine Discovery Institute, Department of Biochemistry and Molecular Biology, Monash University
- Bart W. Hoogenboom
- London Centre for Nanotechnology, University College London
- Ilia Voskoboinik
- Cancer Immunology Program, Peter MacCallum Cancer Centre
- Yann Gambin
- School of Medical Science, University of New South Wales
- Emma Sierecki
- School of Medical Science, University of New South Wales
- Michelle A. Dunstone
- ARC Centre of Excellence in Advanced Molecular Imaging, Monash University
- James C. Whisstock
- ARC Centre of Excellence in Advanced Molecular Imaging, Monash University
- DOI
- https://doi.org/10.1038/s41467-019-12279-2
- Journal volume & issue
-
Vol. 10,
no. 1
pp. 1 – 9
Abstract
Macrophage-expressed gene 1 (MPEG1) functions within the phagolysosome to damage engulfed microbes, presumably via forming pores in target membranes. In order to provide insights into the mechanism of MPEG1 function and membrane binding, the authors present structures of hexadecameric MPEG1 prepores both in solution and in complex with liposomes.
