Use of Melatonin/Decorticotomy and Autogenous Bone Graft in Induced 1-Wall Defect

Hala H. Hazzaa; Mai S. Attia; Marwa A.M. El Shiekh; Mohammed E. Grawish; Mohamed Moawed I. Ghoneim; Nahed M. Adly; Noha S. Shams; Mohamed A. El-Mahdy; Gasser M. Elewa

International Dental Journal (Aug 2023)

Use of Melatonin/Decorticotomy and Autogenous Bone Graft in Induced 1-Wall Defect

Hala H. Hazzaa,
Mai S. Attia,
Marwa A.M. El Shiekh,
Mohammed E. Grawish,
Mohamed Moawed I. Ghoneim,
Nahed M. Adly,
Noha S. Shams,
Mohamed A. El-Mahdy,
Gasser M. Elewa

Affiliations

Hala H. Hazzaa: Professor of Oral Medicine, Periodontology and Oral Diagnosis, Faculty of Dental Medicine for Girls, Al-Azhar University, Cairo, Egypt; Professor of Oral Medicine, Periodontology and Oral Diagnosis, Faculty of Oral and Dental Medicine, Delta University for Science and Technology, Dakahlia, Egypt; Corresponding author. Mai S. Attia, Professor of Oral Medicine, Periodontology, and Oral Diagnosis, Faculty of Dental Medicine for girls, Al-Azhar University, Cairo, Egypt, Faculty of Oral and Dental Medicine, Misr International University,Cairo, Egypt
Mai S. Attia: Professor of Oral Medicine, Periodontology and Oral Diagnosis, Faculty of Oral and Dental Medicine, Delta University for Science and Technology, Dakahlia, Egypt; Professor of Oral Medicine, Periodontology and Oral Diagnosis, Faculty of Oral and Dental Medicine, Misr International University, Cairo, Egypt
Marwa A.M. El Shiekh: Associate Professor of Oral and Dental Biology, Faculty of Dental Medicine for Girls, Al-Azhar University, Cairo, Egypt
Mohammed E. Grawish: Professor of Oral Biology, Faculty of Dentistry, Mansoura University, Egypt; Professor of Oral Biology, Faculty of Oral and Dental Medicine, Delta University for Science and Technology, Dakahlia, Egypt
Mohamed Moawed I. Ghoneim: Associate Professor of Oral and Maxillofacial Surgery, Faculty of Dentistry, Sinai University, Egypt
Nahed M. Adly: Associate Professor of Oral and Maxillofacial Surgery, Faculty of Dental Medicine Al-Azhar University, Cairo, Egypt, Faculty of Dentistry, Al Nahda University, Benisuef, Egypt
Noha S. Shams: Lecturer of Oral pathology, Faculty of Oral and dental Medicine, Ahram Canadian University, 6th of October, Egypt
Mohamed A. El-Mahdy: Lecturer of Oral and Maxillofacial Surgery, Faculty of Dentistry , Al Nahda University, Benisuef, Egypt.
Gasser M. Elewa: Lecturer of Laser Applications in Dentistry, Faculty of Oral and Dental Medicine, Delta University for Science and Technology, Dakahlia, Egypt

Journal volume & issue: Vol. 73, no. 4
pp. 524 – 532

Abstract

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ABSTRACT: Purpose: This study was designed to investigate the effect of intramarrow penetration (IMP) and 1% melatonin (MLN) gel on the remodelling process of autogenous bone graft (ABG) in an induced 1–osseous wall defect model. Methods: Sixty-four intrabony induced mandibular defects were created on the distal side of premolars—P1, P2, P3, and P4 (on each side)—in 8 beagle dogs. A ligature-induced periodontitis was initiated in each defect. Defects were then divided into 4 equal groups. Group I was treated with open-flap debridement (OFD) alone, group II was treated with OFD/ABG, group III was treated with OFD/IMP/ABG, and group IV was treated with OFD/ABG/IMP/1% MLN gel. The study parameters were bone fill, histologic analysis, and immunohistochemical evaluation of endothelial nitric oxide synthase (eNOS) expression at 2-week (2W) and 8-week (8W) time intervals. Results: At 8W, significant differences were revealed amongst all groups regarding the amount of bone fill and eNOS expressions (P < .001). Bone fill percentages were 55.5%, 22.3%, 16.8%, and 0% in groups IV, III, II, and I, respectively. eNOS expressions were 1.68 ± 0.06, 8.43 ± 0.04, 16.80 ± 0.17, and 1.97 ± 0.07 in groups IV, III, II, and I, respectively. The favourable results were in line with group IV. Conclusions: According to these preliminary results, defects treated by ABG augmented with IMP and 1% MLN gel revealed a greater amount of bone fill and reduced eNOS expression. This combination is therefore highly suggested as an adjunct to ABG.

Published in International Dental Journal

ISSN: 0020-6539 (Print); 1875-595X (Online)
Publisher: Elsevier
Country of publisher: United Kingdom
LCC subjects: Medicine: Dentistry
Website: https://www.journals.elsevier.com/international-dental-journal

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