Remedial effects of casticin as an antioxidant on cisplatin induced oxidative damage in rat liver

Abstract

Cisplatin (CP) is an active cytotoxic agent, which has been verified to be effective in multiple cancer regimen. In this study, potential antioxidant effects of casticin (CAS) were assessed against CP generated oxidative stress in rat liver. “Twenty-four male Sprague Dawley rats were divided into four experimental groups. Group-1 (control group) received only normal saline”. Group-2 was intraperitoneally injected with CP (10 mg/kg). Group-3 was orally provided by CAS (50 mg/kg) along with CP (10 mg/kg) injection at first day. Group-4 group was orally administered with CAS (50 mg/kg) throughout the experiment. The rats of group-1 indicated “increase in serum levels of aspartate aminotransferase (AST), alanine aminotransferase (ALT) and alkaline phosphatase (ALP), while a significant decrease in the activities of catalase (CAT), superoxide dismutase (SOD) and peroxidase (POD)” was noticed, which revealed that CP generated oxidative stress in rat liver. Moreover, administration of CP increased the hydrogen peroxide (H2O2) concentration and level of “thiobarbituric acid reactive substances (TBARS)”, while reducing the % DNA head and head length in liver cell nuclei. CP disturbs the lobular structure of the liver and increased the sinusoidal dilation in rat liver. However, co-treatment with CAS successfully mitigated the CP generated DNA disruption, biochemical and pathological changes in rat liver. These findings revealed that an effective antioxidant, CAS, alleviated the CP generated hepatotoxicity and oxidative stress in rats. Keywords: Cisplatin, Oxidative stress, DNA damage, Liver damage, Antioxidant, Casticin