Rapid, Label-Free Prediction of Antibiotic Resistance in <i>Salmonella typhimurium</i> by Surface-Enhanced Raman Spectroscopy

Ping Zhang; Xi-Hao Wu; Lan Su; Hui-Qin Wang; Tai-Feng Lin; Ya-Ping Fang; Hui-Min Zhao; Wen-Jing Lu; Meng-Jia Liu; Wen-Bo Liu; Da-Wei Zheng

doi:10.3390/ijms23031356

International Journal of Molecular Sciences (Jan 2022)

Rapid, Label-Free Prediction of Antibiotic Resistance in <i>Salmonella typhimurium</i> by Surface-Enhanced Raman Spectroscopy

Ping Zhang,
Xi-Hao Wu,
Lan Su,
Hui-Qin Wang,
Tai-Feng Lin,
Ya-Ping Fang,
Hui-Min Zhao,
Wen-Jing Lu,
Meng-Jia Liu,
Wen-Bo Liu,
Da-Wei Zheng

Affiliations

Ping Zhang: Faculty of Environment and Life, Beijing International Science and Technology Cooperation Base of Antivirus Drug, Beijing University of Technology, Beijing 100124, China
Xi-Hao Wu: Faculty of Environment and Life, Beijing International Science and Technology Cooperation Base of Antivirus Drug, Beijing University of Technology, Beijing 100124, China
Lan Su: Faculty of Environment and Life, Beijing International Science and Technology Cooperation Base of Antivirus Drug, Beijing University of Technology, Beijing 100124, China
Hui-Qin Wang: Faculty of Environment and Life, Beijing International Science and Technology Cooperation Base of Antivirus Drug, Beijing University of Technology, Beijing 100124, China
Tai-Feng Lin: Faculty of Environment and Life, Beijing International Science and Technology Cooperation Base of Antivirus Drug, Beijing University of Technology, Beijing 100124, China
Ya-Ping Fang: Faculty of Environment and Life, Beijing International Science and Technology Cooperation Base of Antivirus Drug, Beijing University of Technology, Beijing 100124, China
Hui-Min Zhao: Faculty of Environment and Life, Beijing International Science and Technology Cooperation Base of Antivirus Drug, Beijing University of Technology, Beijing 100124, China
Wen-Jing Lu: Faculty of Environment and Life, Beijing International Science and Technology Cooperation Base of Antivirus Drug, Beijing University of Technology, Beijing 100124, China
Meng-Jia Liu: Faculty of Environment and Life, Beijing International Science and Technology Cooperation Base of Antivirus Drug, Beijing University of Technology, Beijing 100124, China
Wen-Bo Liu: Faculty of Environment and Life, Beijing International Science and Technology Cooperation Base of Antivirus Drug, Beijing University of Technology, Beijing 100124, China
Da-Wei Zheng: Faculty of Environment and Life, Beijing International Science and Technology Cooperation Base of Antivirus Drug, Beijing University of Technology, Beijing 100124, China

DOI: https://doi.org/10.3390/ijms23031356
Journal volume & issue: Vol. 23, no. 3
p. 1356

Abstract

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The rapid identification of bacterial antibiotic susceptibility is pivotal to the rational administration of antibacterial drugs. In this study, cefotaxime (CTX)-derived resistance in Salmonella typhimurium (abbr. CTXr-S. typhimurium) during 3 months of exposure was rapidly recorded using a portable Raman spectrometer. The molecular changes that occurred in the drug-resistant strains were sensitively monitored in whole cells by label-free surface-enhanced Raman scattering (SERS). Various degrees of resistant strains could be accurately discriminated by applying multivariate statistical analyses to bacterial SERS profiles. Minimum inhibitory concentration (MIC) values showed a positive linear correlation with the relative Raman intensities of I990/I1348, and the R2 reached 0.9962. The SERS results were consistent with the data obtained by MIC assays, mutant prevention concentration (MPC) determinations, and Kirby-Bauer antibiotic susceptibility tests (K-B tests). This preliminary proof-of-concept study indicates the high potential of the SERS method to supplement the time-consuming conventional method and help alleviate the challenges of antibiotic resistance in clinical therapy.

Published in International Journal of Molecular Sciences

ISSN: 1661-6596 (Print); 1422-0067 (Online)
Publisher: MDPI AG
Country of publisher: Switzerland
LCC subjects: Science: Biology (General); Science: Chemistry
Website: http://www.mdpi.com/journal/ijms

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