Development of Cytoplasmatic SSR-Markers for Population Genetic Studies of the Siberian Stone Pine (Pinus Sibirica Du Tour)

Abstract

Three chloroplast and one mitochondrial DNA markers were developed and used for genotyping of 60 trees in two populations of the Siberian stone pine (Pinus sibirica Du Tour). Two chloroplast loci were monomorphic in both populations, and one polymorphic with two alleles. Therefore, four chloroplast haplotypes were revealed totally. A mitochondrial DNA marker had two alleles or haplotypes (mitotypes).

Keywords

• DNA markers
• PCR
• chloroplast and mitochondrial markers
• mitotype
• locus
• allele
• haplotype
• Siberian stone pine (Pinus sibirica Du Tour)