Search and processing of Holliday junctions within long DNA by junction-resolving enzymes

Artur P. Kaczmarczyk; Anne-Cécile Déclais; Matthew D. Newton; Simon J. Boulton; David M. J. Lilley; David S. Rueda

doi:10.1038/s41467-022-33503-6

Nature Communications (Oct 2022)

Search and processing of Holliday junctions within long DNA by junction-resolving enzymes

Artur P. Kaczmarczyk,
Anne-Cécile Déclais,
Matthew D. Newton,
Simon J. Boulton,
David M. J. Lilley,
David S. Rueda

Affiliations

Artur P. Kaczmarczyk: Department of Infectious Disease, Faculty of Medicine, Imperial College London
Anne-Cécile Déclais: School of Life Sciences, University of Dundee
Matthew D. Newton: Department of Infectious Disease, Faculty of Medicine, Imperial College London
Simon J. Boulton: DSB Repair Metabolism Laboratory, The Francis Crick Institute
David M. J. Lilley: School of Life Sciences, University of Dundee
David S. Rueda: Department of Infectious Disease, Faculty of Medicine, Imperial College London

DOI: https://doi.org/10.1038/s41467-022-33503-6
Journal volume & issue: Vol. 13, no. 1
pp. 1 – 13

Abstract

Read online

Locating a four-way junction in a high background of genomic DNA is likely to be the rate-limiting step of the resolution process. This study captures the entire reaction trajectory of a nuclease targeting and resolving a DNA junction at single-molecule level.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

About the journal