Chemical and Biological Technologies in Agriculture (Sep 2024)

Lignin synthesis pathway in response to Rhizoctonia solani Kühn infection in potato (Solanum tuberosum L.)

  • XinYu Yang,
  • Wangjun Zhang,
  • HePing Lv,
  • YanPing Gao,
  • YiChen Kang,
  • YanBin Wu,
  • FangFang Wang,
  • Wu Zhang,
  • HongJie Liang

DOI
https://doi.org/10.1186/s40538-024-00663-0
Journal volume & issue
Vol. 11, no. 1
pp. 1 – 11

Abstract

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Abstract Potato black scurf caused by Rhizoctonia solani Kühn is widespread worldwide. The exploration and analysis of the infection mechanism of Rhizoctonia solani Kühn has important scientific significance to enhance the disease resistance of potato and other horticultural crops, and then break the restriction of fungal harm to agricultural production. The physiological and biochemical indexes and the expression levels of related genes were measured at 0, 1, 4, 8 and 16 days (T0, T1, T2, T3, T4) after inoculation with pathogenic bacteria. The results showed that the contents of L-phenylalanine ammonia-lyase (PAL), peroxidase (POD), lignin, total phenols (TP), and flavonoids increased significantly in potato after infection by Rhizoctonia solani Kühn, with the contents of PAL and POD reaching a peak at 8 d and then decreasing, and the contents of lignin and total phenols changing most significantly, reaching the highest levels at day 8 (T3) and day 16 (T4), respectively. During the infestation, the content of eight phenolic compounds increased, and the genes responsible for the lignin synthesis pathway were upregulated. However, in the later stage of infestation, the expression of two genes (PAL PG0031457 and PG2021549, HCT PG0014959, and COMT PG0011266) was down-regulated. In the correlation analysis, gene expression levels of all the genes, except POD (PG0005062), CCoAOMT (PG0018688), and COMT (PG0011266), were found to be positively correlated with the contents of lignin, total phenols, flavonoids, PAL, POD, and eight phenolic substances. Therefore, based on a sound understanding of the occurrence mechanism of Potato black scurf, this experiment analyzed the effect of Rhizoctonia solani Kühn infestation on the content of relevant metabolites in the lignin synthesis pathway as well as gene expression in potatoes, which provides a scientific basis for the prevention and control management of potato black scurf. Graphical Abstract

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