Inhibitory Activity of Shrimp Waste Extracts on Fungal and Oomycete Plant Pathogens

Soumia El boumlasy; Federico La Spada; Nunzio Tuccitto; Giovanni Marletta; Carlos Luz Mínguez; Giuseppe Meca; Ermes Ivan Rovetto; Antonella Pane; Abderrahmane Debdoubi; Santa Olga Cacciola

doi:10.3390/plants10112452

Plants (Nov 2021)

Inhibitory Activity of Shrimp Waste Extracts on Fungal and Oomycete Plant Pathogens

Soumia El boumlasy,
Federico La Spada,
Nunzio Tuccitto,
Giovanni Marletta,
Carlos Luz Mínguez,
Giuseppe Meca,
Ermes Ivan Rovetto,
Antonella Pane,
Abderrahmane Debdoubi,
Santa Olga Cacciola

Affiliations

Soumia El boumlasy: Laboratory of Materials-Catalysis, Chemistry Department, Faculty of Science, University Abdelmalek Essaadi, Tetouan B.P. 2117, Morocco
Federico La Spada: Department of Agriculture, Food and Environment, University of Catania, 95123 Catania, Italy
Nunzio Tuccitto: Consorzio per lo Sviluppo dei Sistemi a Grande Interfase, CSGI, Viale A. Doria 6, 95125 Catania, Italy
Giovanni Marletta: Consorzio per lo Sviluppo dei Sistemi a Grande Interfase, CSGI, Viale A. Doria 6, 95125 Catania, Italy
Carlos Luz Mínguez: Laboratory of Food Chemistry and Toxicology, Faculty of Pharmacy, University of Valencia, Av. Vicent Andrés Estellés s/n, 46100 Burjassot, Spain
Giuseppe Meca: Laboratory of Food Chemistry and Toxicology, Faculty of Pharmacy, University of Valencia, Av. Vicent Andrés Estellés s/n, 46100 Burjassot, Spain
Ermes Ivan Rovetto: Department of Agriculture, Food and Environment, University of Catania, 95123 Catania, Italy
Antonella Pane: Department of Agriculture, Food and Environment, University of Catania, 95123 Catania, Italy
Abderrahmane Debdoubi: Laboratory of Materials-Catalysis, Chemistry Department, Faculty of Science, University Abdelmalek Essaadi, Tetouan B.P. 2117, Morocco
Santa Olga Cacciola: Department of Agriculture, Food and Environment, University of Catania, 95123 Catania, Italy

DOI: https://doi.org/10.3390/plants10112452
Journal volume & issue: Vol. 10, no. 11
p. 2452

Abstract

Read online

(1) Background: This study was aimed at determining the in vitro inhibitory effect of new natural substances obtained by minimal processing from shrimp wastes on fungi and oomycetes in the genera Alternaria, Colletotrichum, Fusarium, Penicillium, Plenodomus and Phytophthora; the effectiveness of the substance with the highest in vitro activity in preventing citrus and apple fruit rot incited by P. digitatum and P. expansum, respectively, was also evaluated. (2) Methods: The four tested substances, water-extract, EtOAc-extract, MetOH-extract and nitric-extract, were analyzed by HPLC-ESI-MS-TOF; in vitro preliminary tests were carried out to determine the minimal inhibitory/fungicidal concentrations (MIC and MFC, respectively) of the raw dry powder, EtOAc-extract, MetOH-extract and nitric-extract for each pathogen. (3) Results: in the agar-diffusion-assay, nitric-extract showed an inhibitory effect on all pathogens, at all concentrations tested (100, 75, 50 and 25%); the maximum activity was on Plenodomus tracheiphilus, C. gloeosporioides and Ph. nicotianae; the diameters of inhibition halos were directly proportional to the extract concentration; values of MIC and MFC of this extract for all pathogens ranged from 2 to 3.5%; the highest concentrations (50 to 100%) tested in vivo were effective in preventing citrus and apple fruit molds. (4) Conclusions: This study contributes to the search for natural and ecofriendly substances for the control of pre- and post-harvest plant pathogens.

Published in Plants

ISSN: 2223-7747 (Online)
Publisher: MDPI AG
Country of publisher: Switzerland
LCC subjects: Science: Botany
Website: http://www.mdpi.com/journal/plants

About the journal

Abstract

Keywords