Identification and Analysis of Dysfunctional Genes and Pathways in CD8+ T Cells of Non-Small Cell Lung Cancer Based on RNA Sequencing

Xuefang Tao; Xiaotang Wu; Tao Huang; Deguang Mu

doi:10.3389/fgene.2020.00352

Frontiers in Genetics (May 2020)

Identification and Analysis of Dysfunctional Genes and Pathways in CD8+ T Cells of Non-Small Cell Lung Cancer Based on RNA Sequencing

Xuefang Tao,
Xiaotang Wu,
Tao Huang,
Deguang Mu

Affiliations

Xuefang Tao: Affiliated Hospital of Shaoxing University, Shaoxing, China
Xiaotang Wu: Shanghai Engineering Research Center of Pharmaceutical Translation, Shanghai, China
Tao Huang: Shanghai Institute of Nutrition and Health, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China
Deguang Mu: Department of Respiratory Medicine, Zhejiang Provincial People’s Hospital, People’s Hospital of Hangzhou Medical College, Hangzhou, China

DOI: https://doi.org/10.3389/fgene.2020.00352
Journal volume & issue: Vol. 11

Abstract

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Lung cancer, the most common of malignant tumors, is typically of the non-small cell (NSCLC) type. T-cell-based immunotherapies are a promising and powerful approach to treating NSCLCs. To characterize the CD8+ T cells of non-small cell lung cancer, we re-analyzed the published RNA-Seq gene expression profiles of 36 CD8+ T cell isolated from tumor (TIL) samples and 32 adjacent uninvolved lung (NTIL) samples. With an advanced Monte Carlo method of feature selection, we identified the CD8+ TIL specific expression patterns. These patterns revealed the key dysfunctional genes and pathways in CD8+ TIL and shed light on the molecular mechanisms of immunity and use of immunotherapy.

Published in Frontiers in Genetics

ISSN: 1664-8021 (Online)
Publisher: Frontiers Media S.A.
Country of publisher: Switzerland
LCC subjects: Science: Biology (General): Genetics
Website: http://journal.frontiersin.org/journal/genetics

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Abstract

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