Determination of acetyl coenzyme A. Interference by a contaminant in malate dehydrogenase

Abstract

Spectrophotometric determinations of acetyl CoA with malate dehydrogenase and citrate synthase are likely to overestimate the amount of acetyl CoA in solutions containing acetoacetyl CoA, since commercial preparations of malate dehydrogenase may contain thiolase.

Keywords

• acetoacetyl CoA
• thiolase