Apoptotic PET Imaging of Rat Pulmonary Fibrosis With [F]ML-8

Ying Xiong MM; Dahong Nie BM; Shaoyu Liu PhD; Hui Ma BM; Shu Su BM; Aixia Sun PhD; Jing Zhao MD, PhD; Zhanwen Zhang PhD; Xianhong Xiang MD, PhD; Ganghua Tang MD, PhD

doi:10.1177/1536012118795728

Molecular Imaging (Oct 2018)

Apoptotic PET Imaging of Rat Pulmonary Fibrosis With [F]ML-8

Ying Xiong MM,
Dahong Nie BM,
Shaoyu Liu PhD,
Hui Ma BM,
Shu Su BM,
Aixia Sun PhD,
Jing Zhao MD, PhD,
Zhanwen Zhang PhD,
Xianhong Xiang MD, PhD,
Ganghua Tang MD, PhD

Affiliations

Ying Xiong MM: Guangdong Engineering Research Center for Translational Application of Medical Radiopharmaceuticals and Department of Medical Imaging, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, China
Dahong Nie BM: Guangdong Engineering Research Center for Translational Application of Medical Radiopharmaceuticals and Department of Medical Imaging, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, China
Shaoyu Liu PhD: Guangdong Engineering Research Center for Translational Application of Medical Radiopharmaceuticals and Department of Medical Imaging, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, China
Hui Ma BM: Guangdong Engineering Research Center for Translational Application of Medical Radiopharmaceuticals and Department of Medical Imaging, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, China
Shu Su BM: Guangdong Engineering Research Center for Translational Application of Medical Radiopharmaceuticals and Department of Medical Imaging, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, China
Aixia Sun PhD: Guangdong Engineering Research Center for Translational Application of Medical Radiopharmaceuticals and Department of Medical Imaging, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, China
Jing Zhao MD, PhD: Guangdong Engineering Research Center for Translational Application of Medical Radiopharmaceuticals and Department of Medical Imaging, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, China
Zhanwen Zhang PhD: Guangdong Engineering Research Center for Translational Application of Medical Radiopharmaceuticals and Department of Medical Imaging, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, China
Xianhong Xiang MD, PhD: Guangdong Engineering Research Center for Translational Application of Medical Radiopharmaceuticals and Department of Medical Imaging, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, China
Ganghua Tang MD, PhD: Guangdong Engineering Research Center for Translational Application of Medical Radiopharmaceuticals and Department of Medical Imaging, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, China

DOI: https://doi.org/10.1177/1536012118795728
Journal volume & issue: Vol. 17

Abstract

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Objective: To investigate the value of 2-(3-[ 18 F]fluoropropyl)-2-methyl-malonic acid ([ 18 F]ML-8) positron emission tomography (PET) imaging of rat pulmonary fibrosis. Methods: Male Sprague-Dawley rats were divided into 2 groups, including pulmonary fibrosis model group and control group. The rat model was established by an intratracheal instillation of bleomycin (BLM). Control rats were treated with saline. Positron emission tomography/computed tomography (CT) with [ 18 F]ML-8 or 18 F-fluorodeoxyglucose ([ 18 F]FDG) was performed on 2 groups. After PET/CT imaging, lung tissues were collected for histologic examination. Data were analyzed and comparisons between 2 groups were performed using Student t test. Results: Bleomycin-treated rats showed a higher lung uptake of [ 18 F]ML-8 than control rats ( P < .05). In BLM-treated rats, the lung to muscle relative uptake ratio of [ 18 F]ML-8 was also higher than that of [ 18 F]FDG ( P < .05). Pathological examination showed overproliferation of fibroblasts and deposition of collagen in lungs from BLM-treated rats. Compared to control rats, BLM-treated rats had higher lung hydroxyproline content ( P < .05). Immunofluorescence staining indicated more apoptotic cells in BLM-treated rats than those in control rats. Moreover, the apoptosis rate of lung tissues obtained from BLM-treated rats was higher than that from control rats ( P < .05). Conclusions: 2-(3-[ 18 F]fluoropropyl)-2-methyl-malonic acid PET/CT could be used for noninvasive diagnosis of pulmonary fibrosis in a rat model.

Published in Molecular Imaging

ISSN: 1536-0121 (Online)
Publisher: SAGE Publications
Country of publisher: United States
LCC subjects: Science: Biology (General); Medicine: Medicine (General): Medical technology
Website: https://journals.sagepub.com/home/MIX

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