Bioreduction in vitro of hexavalent chromium using a microbial consortium

Abstract

Constant industrial development has increased the chromium use, resulting in chromium discharge into wastewater. The implementation of microbial bioremediation has been proposed as an ecological, efficient and economical alternative for the implementation of microorganisms to reduce chromium to a less toxic form. Our objective was to evaluate the Cr (VI) removal capacity of microbial consortia at different concentrations. Accordingly, an activation and identification of microorganisms from chromium-containing wastewater was carried out. Seven microbial consortia were established and their synergy, individual and consortium growth curves were evaluated. Subsequently, a scaled adaptation of the consortia was carried out with eight concentrations of Cr (VI). Reciprocal regression models and growth curves were used to identify the consortium with the highest removal. Synergy was found in the consortia evaluated; growth curves of consortia showed higher absorbance than individually, with higher absorption in the Candida famata-Serratia sp. Consortium. However, in the scaled adaptation, greater Cr (VI) reduction capacity was demonstrated in Candida tropicalis-Serratia sp. with 79.20% at a concentration of 100 ppm with a total chromium reduction of 31.12%. At the same time, a greater adaptation of the consortia to high concentrations of Cr (VI) was identified. This is the first research to report C. tropicalis-Serratia sp. microbial consortium with a positive interaction and higher metabolic reduction capacity, which will have a positive impact on bioremediation of chromium-containing wastewater.

Keywords

• bioremediation
• hexavalent chromium
• total chromium
• microbial consortia
• removal