A new polysaccharide from Caulerpa chemnitzia induces molecular shifts of immunomodulation on macrophages RAW264.7

Yulin Wu; Jun Liu; Huili Hao; Lianmei Hu; Xiaoyong Zhang; Lianxiang Luo; Jincheng Zeng; Wei Zhang; Io Nam Wong; Riming Huang

Food Chemistry: X (Jun 2022)

A new polysaccharide from Caulerpa chemnitzia induces molecular shifts of immunomodulation on macrophages RAW264.7

Yulin Wu,
Jun Liu,
Huili Hao,
Lianmei Hu,
Xiaoyong Zhang,
Lianxiang Luo,
Jincheng Zeng,
Wei Zhang,
Io Nam Wong,
Riming Huang

Affiliations

Yulin Wu: Guangdong Provincial Key Laboratory of Food Quality and Safety, College of Food Science, South China Agricultural University, Guangzhou 510642, China
Jun Liu: Laboratory of Pathogenic Biology, The Marine Biomedical Research Institute, Dongguan Key Laboratory of Medical Bioactive Molecular Developmental and Translational Research, Guangdong Medical University, Zhanjiang, Guangdong 524023, China
Huili Hao: Guangdong Provincial Key Laboratory of Food Quality and Safety, College of Food Science, South China Agricultural University, Guangzhou 510642, China
Lianmei Hu: College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China
Xiaoyong Zhang: Joint Laboratory of Guangdong Province and Hong Kong Region on Marine Bioresource Conservation and Exploitation, College of Marine Sciences, South China Agricultural University, Guangzhou 510642, China
Lianxiang Luo: Laboratory of Pathogenic Biology, The Marine Biomedical Research Institute, Dongguan Key Laboratory of Medical Bioactive Molecular Developmental and Translational Research, Guangdong Medical University, Zhanjiang, Guangdong 524023, China
Jincheng Zeng: Laboratory of Pathogenic Biology, The Marine Biomedical Research Institute, Dongguan Key Laboratory of Medical Bioactive Molecular Developmental and Translational Research, Guangdong Medical University, Zhanjiang, Guangdong 524023, China
Wei Zhang: State Key Laboratory of Quality Research in Chinese Medicines and Macau Institute for Applied Research in Medicine and Health, Faculty of Medicine, Macau University of Science and Technology, Taipa, Macau 999078, China
Io Nam Wong: State Key Laboratory of Quality Research in Chinese Medicines and Macau Institute for Applied Research in Medicine and Health, Faculty of Medicine, Macau University of Science and Technology, Taipa, Macau 999078, China
Riming Huang: Guangdong Provincial Key Laboratory of Food Quality and Safety, College of Food Science, South China Agricultural University, Guangzhou 510642, China; Corresponding author.

Journal volume & issue: Vol. 14
p. 100313

Abstract

Read online

Investigation on Caulerpa chemnitzia polysaccharides led to the finding of a new polysaccharide (CCP). The basic components of CCP were the total sugar (59.18% ± 0.57%), the uronic acids (36.75% ± 0.28%) and the sulfate (42.50% ± 0.42%), in total content. The physicochemical analysis revealed that CCP was a heteropolysaccharide with a molecular weight of 321.6 KDa, and composed of arabinose, fucose, glucose, mannose, galactose, xylose, fructose, ribose, glucuronic acid and galacturonic acid. The immunomodulatory assay showed that CCP played an important role in activating cell viability, the nitric oxide product and cytokines (IL-6 and TNF-α) secretion. Furthermore, the transcript-metabolic analysis displayed a total of 7692 differentially expressed genes (DEGs) and 95 differentially accumulated metabolites (DAMs), and revealed that CCP may play an immunomodulatory effect by activating NF-κB signaling pathway and arachidonic acid metabolism pathway. These findings will provide a basic understanding to further investigation of Caulerpa polysaccharides.

Published in Food Chemistry: X

ISSN: 2590-1575 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Technology: Home economics: Nutrition. Foods and food supply; Technology: Chemical technology: Food processing and manufacture
Website: https://www.journals.elsevier.com/food-chemistry-x

About the journal

Abstract

Keywords