The Effect of The Conditioned Medium from Human Embryonic Stem Cells on Mouse Oocytes In Vitro Maturation

Abstract

Objective: Some reports have indicated that conditioned medium from growing mouse embryonic stem cells (ESCs)provides a supportive condition for small follicles growing, oocyte maturation, and following embryo growth. The aim ofthis study is assessing in vitro maturation (IVM) and consequent in vitro fertilization (IVF) outcome of immature mouseoocytes using human embryonic stem cells conditioned medium (HESCM).Materials and Methods: In this experimental study, 240 germinal vesicle (GV) oocytes were took from NMRI femalemice, aged 4-6 weeks, 48 hours before injection of 5 IU pregnant mare serum gonadotropin (PMSG). 120 GV oocyteswithout cumulus cells were cultured in each of the groups. 120 GV were cultured in HESCM as test groups and also120 GV cultured in human embryonic stem cells medium (HESM) as control groups. After evaluating the metaphase II(MII) oocyte maturation rate at 8, 16 and 24 hours, the MII oocytes subsequently were fertilized in vitro and the two-cellembryo development rate was recorded at days 1, 2, and 3. Statistical analysis was performed by using the generalizedestimating equations (GEE) method that calculated their rate ratio.Results: Our data indicated there are significant differences between the maturation rates in HESCM and HESM(P=0.004), also the two-cell embryo development was significant between two culture media (P=0.00).Conclusion: Similar to some other studies, the secretome of the HESCM showed a significant impact on the IVMoutcomes in mice.

Keywords

• assisted reproductive techniques
• conditioned medium
• germinal vesicle
• human embryonic stem cells
• in vitro maturation